Hematopoietic- and neurologic-expressed sequence 1 expression in the murine GL261 and high-grade human gliomas.
Hematopoietic- and neurologic-expressed sequence 1 expression in the murine GL261 and high-grade human gliomas.
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DOI：
10.1007/s12253-008-9147-4
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发表时间：
2009-09
影响因子：
2.8
通讯作者：
Harrison, Jeffrey K.
中科院分区：
文献类型：
--
作者：
Laughlin, Katharine M.;Luo, Defang;Liu, Che;Shaw, Gerry;Warrington, Kenneth H., Jr.;Qiu, Jingxin;Yachnis, Anthony T.;Harrison, Jeffrey K.
造血和神经表达序列1 （Hn1）基因编码一种在发育和再生组织中表达的高度保守的蛋白质。本研究评估了Hn1在人和小鼠恶性胶质瘤中的表达。在小鼠GL261胶质瘤细胞系和GL261脑肿瘤组织中均检测到Hn1 mRNA和蛋白表达。HN1在人U118MG和U87MG细胞系中也有表达。用抗hn1多克隆抗体对人脑肿瘤进行评价，在高级别（WHO III和IV级）恶性胶质瘤中检测到强免疫反应性。使用抗Hn1 siRNA去除Hn1后，GL261细胞的体外增殖率未发生变化。然而，从hn1缺失的GL261细胞中建立的肿瘤形成的体积明显小于从对照处理的细胞中建立的肿瘤。这些数据表明Hn1在恶性脑肿瘤的生物学中起作用。
The hematopoietic- and neurologic-expressed sequence 1 (Hn1) gene encodes a highly conserved protein that is expressed in developing and regenerating tissues. In this study, Hn1 expression was evaluated in human and murine malignant gliomas. Hn1 mRNA and protein were detected in the murine GL261 glioma cell line and in GL261 brain tumors in vivo. HN1 is also expressed in human U118MG and U87MG cell lines. Evaluation of human brain tumors using an anti-Hn1 polyclonal antibody detected strong immunoreactivity in high-grade (WHO III and IV) malignant gliomas. The rate of GL261 cell proliferation in vitro was unaltered by Hn1 depletion using an anti-Hn1 siRNA. However, tumors established from Hn1-depleted GL261 cells formed significantly smaller volumes than those established from control-treated cells. These data suggest a role for Hn1 in the biology of malignant brain tumors.
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影响因子：
2.5
作者：
Tang, WZ;Lai, YH;Chui, DHK
通讯作者：
Chui, DHK
影响因子：
5.1
作者：
McCarty, DM;Monahan, PE;Samulski, RJ
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影响因子：
64.8
作者：
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通讯作者：
VOGELSTEIN, B
影响因子：
3.3
作者：
Liu, Che;Luo, Defang;Harrison, Jeffrey K.
通讯作者：
Harrison, Jeffrey K.
影响因子：
4.8
作者：
Harrison, JK;Luo, DF;Streit, WJ
通讯作者：
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