The Trichoplusia ni single nucleopolyhedrovirus tn79 gene encodes a functional sulfhydryl oxidase enzyme that is able to support the replication of Autographa californica multiple nucleopolyhedrovirus lacking the sulfhydryl oxidase ac92 gene.
The Trichoplusia ni single nucleopolyhedrovirus tn79 gene encodes a functional sulfhydryl oxidase enzyme that is able to support the replication of Autographa californica multiple nucleopolyhedrovirus lacking the sulfhydryl oxidase ac92 gene.
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DOI：
10.1016/j.virol.2014.05.006
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发表时间：
2014-07
期刊：
影响因子：
3.7
通讯作者：
Passarelli, A. Lorena
中科院分区：
文献类型：
--
作者：
Clem, Stian A.;Wu, Wenbi;Passarelli, A. Lorena
加州自签名多核多角体病毒ac92是一个保守的杆状病毒基因，与黄素腺嘌呤二核苷酸连接的巯基氧化酶同源。其产物Ac92是一种功能性巯基氧化酶。ac92的缺失导致芽病毒（BV）产生几乎可以忽略不计的水平，闭塞性衍生病毒（ODV）共包膜缺陷以及它们在闭塞体中的低效整合。为了确定巯基氧化在BV的产生、核衣壳的包膜和核衣壳并入闭塞体中的作用，用单核多角体病毒同源物Tn79代替ac92。Tn79被发现是一种活性巯基氧化酶，它取代Ac92，导致产生感染性BV，尽管比含Ac92的病毒少约10倍。Tn79修复了由于缺乏ac92而导致的ODV形态发生缺陷。活跃的Tn79巯基氧化酶活性是有效的BV产生、ODV包膜以及在缺乏ac92的情况下它们随后并入闭塞体所必需的。
The Autographa californica multiple nucleopolyhedrovirus ac92 is a conserved baculovirus gene with homology to flavin adenine dinucleotide-linked sulfhydryl oxidases. Its product, Ac92, is a functional sulfhydryl oxidase. Deletion of ac92 results in almost negligible levels of budded virus (BV) production, defects in occlusion-derived virus (ODV) co-envelopment and their inefficient incorporation into occlusion bodies. To determine the role of sulfhydryl oxidation in the production of BV, envelopment of nucleocapsids, and nucleocapsid incorporation into occlusion bodies, the Trichoplusia ni single nucleopolyhedrovirus ortholog, Tn79, was substituted for ac92. Tn79 was found to be an active sulfhydryl oxidase that substituted for Ac92, resulting in the production of infectious BV, albeit about 10-fold less than an ac92-containing virus. Tn79 rescued defects in ODV morphogenesis caused by a lack of ac92. Active Tn79 sulfhydryl oxidase activity is required for efficient BV production, ODV envelopment, and their subsequent incorporation into occlusion bodies in the absence of ac92.
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