Voluntary running exercise modifies astrocytic population and features in the peri-infarct cortex.
Voluntary running exercise modifies astrocytic population and features in the peri-infarct cortex.
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DOI：
10.1016/j.ibneur.2023.02.004
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发表时间：
2023-06
影响因子：
1.5
通讯作者：
Tanaka, Hidekazu
中科院分区：
文献类型：
--
作者：
Yamaguchi, Natsumi;Sawano, Toshinori;Nakatani, Jin;Nakano-Doi, Akiko;Nakagomi, Takayuki;Matsuyama, Tomohiro;Tanaka, Hidekazu
脑卒中后的反射运动对神经元的形态可塑性有有益的影响。特别地，局灶性脑缺血后的自主跑步运动促进功能恢复并改善梗死周围运动皮质层5中缺血诱导的树突棘损失。此外，神经元形态受到神经元周围环境变化的影响。神经胶质细胞，其表型可以通过运动改变，已知在这种神经元周围环境的形成中发挥关键作用。在此，我们研究了自愿跑步运动对大脑中动脉闭塞后胶质细胞的影响。自愿跑步运动增加了梗死周围皮质中术后0天（POD）至3天（POD 15）出生的胶质细胞酸性蛋白阳性星形胶质细胞的数量。运动后，缺血后星形胶质细胞的转录组学分析显示10个上调和70个下调基因。此外，基因本体分析表明，下调的70个基因与神经元形态学显著相关。此外，运动减少了表达脂质运载蛋白2的星形胶质细胞的数量，脂质运载蛋白2是一种树突棘密度的调节剂，在POD 15。我们的研究结果表明，运动改变了星形胶质细胞群体的组成和表型。
Rehabilitative exercise following a brain stroke has beneficial effects on the morphological plasticity of neurons. Particularly, voluntary running exercise after focal cerebral ischemia promotes functional recovery and ameliorates ischemia-induced dendritic spine loss in the peri-infarct motor cortex layer 5. Moreover, neuronal morphology is affected by changes in the perineuronal environment. Glial cells, whose phenotypes may be altered by exercise, are known to play a pivotal role in the formation of this perineuronal environment. Herein, we investigated the effects of voluntary running exercise on glial cells after middle cerebral artery occlusion. Voluntary running exercise increased the population of glial fibrillary acidic protein-positive astrocytes born between post-operative days (POD) 0 and 3 on POD15 in the peri-infarct cortex. After exercise, transcriptomic analysis of post-ischemic astrocytes revealed 10 upregulated and 70 downregulated genes. Furthermore, gene ontology analysis showed that the 70 downregulated genes were significantly associated with neuronal morphology. In addition, exercise reduced the number of astrocytes expressing lipocalin 2, a regulator of dendritic spine density, on POD15. Our results suggest that exercise modifies the composition of astrocytic population and their phenotype.
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影响因子：
2.9
作者：
Kopp BL;Wick D;Herman JP
通讯作者：
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