Fgk3 glycogen synthase kinase is important for development, pathogenesis, and stress responses in Fusarium graminearum.
Fgk3 glycogen synthase kinase is important for development, pathogenesis, and stress responses in Fusarium graminearum.
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Fgk3 糖原合酶激酶对于禾谷镰刀菌的发育、发病机制和应激反应很重要
DOI：
10.1038/srep08504
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发表时间：
2015-02-23
影响因子：
4.6
通讯作者：
Wang C
中科院分区：
文献类型：
--
作者：
Qin J;Wang G;Jiang C;Xu JR;Wang C
小麦赤霉病是一种重要的小麦病害。在先前的研究中，与哺乳动物GSK3同源的FGK3糖原合成酶激酶基因被确定为一个重要的毒力因子。虽然GSK3同源物保守性很好，但在真菌病原体中没有一个具有功能特征。在这项研究中，我们进一步表征了FGK3基因的作用。Δfgk3突变体在生长速度、分生孢子形态、萌发和包被形成方面存在多效性缺陷。在感染试验中无致病性，并阻断DON的产生。Δfgk3突变体的糖原积累增加，证实了Fgk3对糖原合成酶的抑制作用。在FGK3-GFP转化体中，GFP信号主要定位于分生孢子的细胞质，而在菌丝中则定位于细胞质和细胞核。此外，FGK3的表达水平在低温、H2O2和SDS胁迫下升高。在Δfgk3突变体中，冷、热、盐胁迫均未能诱导应激反应相关基因FgGRE2、FgGPD1、FgCTT1和FgMSN2的表达。在80 mM LiCl（一种GSK3激酶抑制剂）存在下，野生型表现出与Δfgk3突变体相似的缺陷。总的来说，我们的研究结果表明FGK3对禾粒镰刀菌的生长、条件发生、DON的产生、致病性和胁迫反应都很重要。
Wheat scab caused by Fusarium graminearum is an important disease. In a previous study, the FGK3 glycogen synthase kinase gene orthologous to mammalian GSK3 was identified as an important virulence factor. Although GSK3 orthologs are well-conserved, none of them have been functionally characterized in fungal pathogens. In this study, we further characterized the roles of FGK3 gene. The Δfgk3 mutant had pleiotropic defects in growth rate, conidium morphology, germination, and perithecium formation. It was non-pathogenic in infection assays and blocked in DON production. Glycogen accumulation was increased in the Δfgk3 mutant, confirming the inhibitory role of Fgk3 on glycogen synthase. In FGK3-GFP transformants, GFP signals mainly localized to the cytoplasm in conidia but to the cytoplasm and nucleus in hyphae. Moreover, the expression level of FGK3 increased in response to cold, H2O2, and SDS stresses. In the Δfgk3 mutant, cold, heat, and salt stresses failed to induce the expression of the stress response-related genes FgGRE2, FgGPD1, FgCTT1, and FgMSN2. In the presence of 80 mM LiCl, a GSK3 kinase inhibitor, the wild type displayed similar defects to the Δfgk3 mutant. Overall, our results indicate that FGK3 is important for growth, conidiogenesis, DON production, pathogenicity, and stress responses in F. graminearum.
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