Characterization of a MH2 mutant lacking the v-myc oncogene.
Characterization of a MH2 mutant lacking the v-myc oncogene.
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缺乏 v-myc 癌基因的 MH2 突变体的表征。
DOI：
10.1016/0042-6822(86)90030-9
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发表时间：
1986
期刊：
影响因子：
3.7
通讯作者：
S. Saule
中科院分区：
文献类型：
--
作者：
P. Martin;C. Henry;F. Denhez;P. Amouyel;C. Béchade;G. Calothy;B. Debuire;D. Stéhelin;S. Saule
我们此前曾报道，从鸡神经视网膜细胞传代的野生型(Wt)MH2库中可以分离到一种缺乏转化鹌鹑胚胎细胞能力的MH2-PA200病毒。我们在这里报告了这个MH2-PA200前病毒的分子克隆和广泛的特征。通过转染实验发现分子克隆的MH2-PA200DNA能够刺激视网膜神经细胞的生长，我们的结果表明该重组病毒来源于RAV-1辅助病毒，其中以辅助(ΔGag-Poll-Δenv)序列为代价获得了MH2的V-My和一小部分V-mycof MH2。为了确定环状病毒基因之间的精确边界，我们测定了连接片段的核苷酸序列，结果表明，在重组点，该环状基因的13个核苷酸中有11个与真菌序列完全一致。另一个相似的独立产生的MH2突变体的myc-env连接片段的核苷酸序列显示，在发生在另一个位点的重组点上，环境和真菌序列之间有9个核苷酸的同源性，这表明MH2和RAV-1病毒之间发生了同源重组，产生了MH2-PA200和类似的突变体。
We have previously reported that a virus, MH2-PA200, lacking the ability to transform quail embryo cells, could be isolated from wild type (wt) MH2 stocks passaged on chicken neuroretina cells. We report here the molecular cloning and extensive characterization of this MH2-PA200 provirus. Molecularly cloned MH2-PA200 DNA was found to stimulate the growth of neuroretina cells by transfection assays and our results indicate that this recombinant virus was derived from the RAV-1 helper virus, in whichv-miland a small part ofv-mycof MH2 were acquired at the expense of helper (Δgag-pol-Δenv) sequences. In order to assess the precise boundary between themycandenvgenes we determined the nucleotide sequence of the junction fragment and showed that 11 of 13 nucleotides of theenvgene were identical to themycsequence at the recombination point. The nucleotide sequence of themyc-envjunction fragment of another similar and independently generated MH2 mutant showed similarly 9 nucleotides of homology between theenvandmycsequences at the recombination point that took place at another site, suggesting that a homologous recombination occurred between MH2 and RAV-1 viruses to generate MH2-PA200 and similar mutants.
禽癌病毒MH2的核苷酸序列：两个潜在的onc基因，一个与禽病毒MC29相关，另一个与鼠肉瘤病毒3611相关。
DOI：
10.1073/pnas.81.10.3000
发表时间：
1984
影响因子：
11.1
作者：
Kan,NC;Flordellis,CS;Mark,GE;Duesberg,PH;Papas,TS
通讯作者：
Papas,TS