Experimental gastritis induced by Helicobacter pylori in Japanese monkeys
Experimental gastritis induced by Helicobacter pylori in Japanese monkeys
复制标题
日本猴幽门螺杆菌诱发实验性胃炎
作者：
R. Shuto;Toshio Fujioka;T. Kubota;Masaru Nasu;Second
我们使用日本猴（Macaca fuscata）建立实验模型，以阐明幽门螺杆菌在胃和十二指肠疾病中的致病性。将H.用内窥镜将从人分离的幽门螺杆菌细胞喷洒在17只动物中的12只动物的胃窦周围。其余5只动物未接种;作为对照组。在接种后第7、14和28天，对胃粘膜样品进行大体检查，并用内窥镜进行活组织检查以进行显微镜检查。H.幽门螺杆菌从12只接种动物中的7只（58%）中恢复，并且在组织学上观察到中性粒细胞和单核细胞的浸润。在其中5只动物中观察到肉眼可见的胃炎伴红斑和糜烂。接种后第28天，感染组中的5只动物接受氨苄青霉素治疗。在两只感染但未经治疗的动物中，细菌持续存在超过6个月。感染组的胃窦粘膜胃炎评分和胃液氨浓度显著高于对照组（P < 0.01至0.001），但在氨苄青霉素治疗后，这些值下降至与对照组相当的水平。7天后，感染组7只动物中的5只动物的胃活检样本中的尿素酶活性呈阳性，14天后，其中4只动物的胃活检样本中的尿素酶活性呈阳性，但对照组动物的所有样本中的尿素酶活性均呈阴性。感染组血清中抗体（免疫球蛋白G）水平升高，而对照组变化很小。这些结果表明，该M. fuscata模型可用于H. pylori感染和H. pylori可诱发胃炎。
We used Japanese monkeys (Macaca fuscata) to establish an experimental model in order to clarify the pathogenicity of Helicobacter pylori in gastric and duodenal disorders. A suspension (5 ml; 10(9) CFU/ml) of H. pylori cells isolated from humans was sprayed around the antrum of the stomach of each of 12 of 17 animals with an endoscope. The remaining five animals were not inoculated; they served as a control group. On days 7, 14, and 28 after inoculation, the gastric mucosa samples were examined grossly and were biopsied for microscopic examination with an endoscope. H. pylori was recovered from 7 of the 12 inoculated animals (58%), and infiltration by neutrophils and monocytes was observed histologically. Macroscopic gastritis with erythema and erosions were noted for five of these animals. On day 28 after inoculation, five animals in the infected group were treated with ampicillin. In two infected but untreated animals, the bacteria persisted for more than 6 months. The result of the gastritis scoring of the antral mucosa and the ammonia concentration in the gastric secretion were significantly higher (P < 0.01 to 0.001) for the infected group than for the control group; however, these values decreased to levels comparable to those for the control group after treatment with ampicillin. Urease activity was positive in gastric biopsy specimens from five of the seven animals in the infected group after 7 days and from four of these animals after 14 days but was negative in all specimens from animals in the control group. The level of antibody (immunoglobulin G) in serum for the infected group was elevated but changed very little for the control group. These results suggest that this M. fuscata model can be used to study H. pylori infection and that H. pylori can induce gastritis.
幽门螺杆菌在常规仔猪中的持续存在。
DOI：
10.1093/infdis/161.6.1299
发表时间：
1990
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Eaton,KA;Morgan,DR;Krakowka,S
通讯作者：
Krakowka,S