Amino-acid sequence of the β-subunit of the (Na+ + K+)ATPase deduced from a cDNA
Amino-acid sequence of the β-subunit of the (Na+ + K+)ATPase deduced from a cDNA
复制标题
从 cDNA 推导的 (Na+ + K+)ATP 酶 β 亚基的氨基酸序列
作者：
G. Shull;L. K. Lane;J. Lingrel
钠/钾依赖的ATP酶[(Na++K+)ATPase]由α和β两个亚基组成，它建立和维持动物细胞质膜上的Na+和K+梯度。编码绵羊肾脏1催化亚单位(α)亚基和鱼雷加利福尼亚电浆2酶的互补克隆已被分离和鉴定。然而，关于β-亚基的一级结构的信息很少，这是一种功能未知的糖蛋白，相对分子质量(MR)∼55,000(参考文献。3)。在这里，我们描述了一个包含绵羊肾脏(Na++K+)β-亚基完整编码区的克隆。我们还讨论了该蛋白质的结构方面，并提出了与大肠杆菌K+-ATPase4的KdpC亚基可能进化关系的证据。
The sodium/potassium-dependent ATPase [(Na+ + K+)ATPase], which establishes and maintains the Na+ and K+ gradients across the plasma membrane of animal cells, consists of two subunits, α and β. Complementary DNA clones encoding the catalytic (α) subunit of sheep kidney1 and Torpedo californica electroplax2 enzymes have previously been isolated and characterized. However, there is little information concerning the primary structure of the β-subunit, a glycoprotein of unknown function and relative molecular mass (Mr) ∼55,000 (ref. 3). Here we describe the isolation and characterization of a cDNA clone containing the entire coding region of the β-subunit of the sheep kidney (Na+ + K+)ATPase. We also discuss structural aspects of the protein and present evidence for a possible evolutionary relationship with the KdpC subunit of the Escherichia coli K+-ATPase4.
羔羊和大鼠肾脏 Na 和 K 依赖性 ATP 酶的异硫氰酸荧光素反应位点的氨基酸序列。
DOI：
10.1016/0006-291x(84)90605-3
发表时间：
1984
影响因子：
3.1
作者：
Kirley,TL;Wallick,ET;Lane,LK
通讯作者：
Lane,LK