Putative excitatory and putative inhibitory inputs are localised in different dendritic domains in a Drosophila flight motoneuron.
Putative excitatory and putative inhibitory inputs are localised in different dendritic domains in a Drosophila flight motoneuron.
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DOI：
10.1111/ejn.12104
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发表时间：
2013-03
期刊：
影响因子：
--
通讯作者：
Duch C
中科院分区：
文献类型：
--
作者：
Kuehn C;Duch C
单个神经元的输入-输出计算可能受到树突三维结构和树突特定部位输入突触的影响。然而，树突结构可以与神经元的行为相关计算相关联的例子很少。通过结合遗传学、免疫组织化学和共聚焦激光扫描方法，本研究估计了一个单独鉴定的果蝇飞行运动神经元MN5上spike启动区的位置和假定的突触输入的树突分布模式。MN5是一个单极神经元，有4000多个树突分支。通过将钠通道免疫标记映射到MN5的几何重构上，估计了刺突起始的位置。通过在MN5树突表面重建上标记d - α7烟碱乙酰胆碱受体和Rdl GABAA受体，绘制了MN5树突上推测的兴奋性胆碱能和推测的抑制性gab碱能输入图。虽然这些方法只提供了假定的输入突触分布的估计，但数据表明，抑制性和兴奋性突触优先针对MN5的不同树突结构域，因此，大多数是单独计算的。大多数假定的抑制输入接近于spike起始，这与先前基于飞行过程中运动神经元放电模式记录的预测一致。相比之下，在更远的树突区域，假设的兴奋性输入的最高密度与飞行过程中不同功率需求的预测一致，飞行运动神经元树突的强直兴奋性驱动必须顺利转化为不同的强直放电频率。
Input-output computations of individual neurons may be affected by the three-dimensional structure of their dendrites and by the targeting of input synapses to specific parts of their dendrites. However, only few examples exist where dendritic architecture can be related to behaviorally relevant computations of a neuron. By combining genetic, immunohistochemical, and confocal laser scanning methods this study estimates the location of the spike initiating zone and the dendritic distribution patterns of putative synaptic inputs on an individually identified Drosophila flight motorneuron, MN5. MN5 is a monopolar neuron with more than 4000 dendritic branches. The site of spike initiation was estimated by mapping sodium channel immunolabel onto geometric reconstructions of MN5. Maps of putative excitatory cholinergic and of putative inhibitory GABAergic inputs on MN5 dendrites were created by charting tagged Dα7 nicotinic acetylcholine receptors and Rdl GABAA receptors onto MN5 dendritic surface reconstructions. Although these methods provided only an estimate of putative input synapse distributions, the data indicated that inhibitory and excitatory synapses were targeted preferentially to different dendritic domains of MN5, and thus, computed mostly separately. Most putative inhibitory inputs were close to spike initiation, which was consistent with sharp inhibition, as predicted previously based on recordings of motoneuron firing patterns during flight. By contrast, highest densities of putative excitatory inputs at more distant dendritic regions were consistent with the prediction that in response to different power demands during flight, tonic excitatory drive to flight motoneuron dendrites must be smoothly translated into different tonic firing frequencies.
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影响因子：
9.8
作者：
Fayyazuddin A;Zaheer MA;Hiesinger PR;Bellen HJ
通讯作者：
Bellen HJ
DOI：
10.1111/j.1749-6632.1998.tb09056.x
发表时间：
1998-01-01
期刊：
NEURONAL MECHANISMS FOR GENERATING LOCOMOTOR ACTIVITY
影响因子：
--
作者：
Baro, DJ;Harris-Warrick, RM
通讯作者：
Harris-Warrick, RM
影响因子：
5.3
作者：
Duch, C;Levine, RB
通讯作者：
Levine, RB
影响因子：
2.7
作者：
Evers, J. F.;Muench, D.;Duch, C.
通讯作者：
Duch, C.
影响因子：
25
作者：
Di Cristo, G;Wu, CZ;Huang, ZJ
通讯作者：
Huang, ZJ