Succinylated chitosan derivative has local protective effects on intestinal inflammation.
Succinylated chitosan derivative has local protective effects on intestinal inflammation.
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DOI：
10.1021/acsbiomaterials.7b00262
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发表时间：
2017
影响因子：
5.8
通讯作者：
Yeo Y
中科院分区：
文献类型：
--
作者：
Hyun H;Hashimoto-Hill S;Kim M;Tsifansky MD;Kim CH;Yeo Y
我们以前曾报道过水溶性壳聚糖衍生物，两性离子壳聚糖（ZWC）的抗炎作用。在本研究中，我们假设口服ZWC将在肠腔中提供局部抗炎作用。ZWC确实在各种体外模型中显示出抗炎作用，包括腹膜巨噬细胞、工程化THP 1单核细胞和Caco-2细胞。在Caco-2细胞中，ZWC在脂多糖（LPS）攻击之前应用比在其之后应用时更有效地防止LPS诱导的细胞损伤。当通过饮用水给予小鼠作为预防措施时，ZWC保护动物免受2，4，6-三硝基苯磺酸（TNBS）诱导的结肠炎，帮助它们恢复体重，恢复结肠的大体和组织学外观，并产生FoxP 3 + T细胞。相反，口服ZWC不能保护动物免受LPS诱导的全身炎症。这些结果表明，口服给药的ZWC通过上胃肠道以最小吸收到达结肠，并提供局部抗炎作用。
We have previously reported on the anti-inflammatory effects of a water-soluble chitosan derivative, zwitterionic chitosan (ZWC). In the present study, we hypothesized that orally-administered ZWC would provide local anti-inflammatory effects in the intestinal lumen. ZWC indeed showed anti-inflammatory effects in various in-vitro models including peritoneal macrophages, engineered THP1 monocytes, and Caco-2 cells. In Caco-2 cells, ZWC applied before the lipopolysaccharide (LPS) challenge was more effective than when it was applied after it in preventing LPS-induced cell damage. When administered to mice via drinking water as a prophylactic measure, ZWC protected the animals from 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis, helping them to recover the body weight, restore the gross and histological appearance of the colon, and generate FoxP3+ T cells. In contrast, orally-administered ZWC did not protect the animals from LPS-induced systemic inflammation. These results indicate that orally-administered ZWC reaches the colon with minimal absorption through the upper gastrointestinal tract and provides a local anti-inflammatory effect.
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影响因子：
4.6
作者：
Cho EJ;Doh KO;Park J;Hyun H;Wilson EM;Snyder PW;Tsifansky MD;Yeo Y
通讯作者：
Yeo Y
影响因子：
10.8
作者：
Chae, SY;Jang, MK;Nah, JW
通讯作者：
Nah, JW
影响因子：
3.4
作者：
Baldrick, Paul
通讯作者：
Baldrick, Paul
影响因子：
5.8
作者：
Mura, C.;Nacher, A.;Diez-Sales, O.
通讯作者：
Diez-Sales, O.
影响因子：
6
作者：
Guo, Shuhong;Al-Sadi, Rana;Ma, Thomas Y.
通讯作者：
Ma, Thomas Y.