Histo-blood group antigens in Crassostrea gigas and binding profiles with GII.4 Norovirus
Histo-blood group antigens in Crassostrea gigas and binding profiles with GII.4 Norovirus
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巨牡蛎的组织血型抗原及其与 GII.4 诺如病毒的结合谱
DOI：
10.1007/s00343-018-7024-x
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发表时间：
2018
影响因子：
1.6
通讯作者：
D. Duan
中科院分区：
文献类型：
--
作者：
Liping Ma;Hui Liu;Laijin Su;Feng Zhao;Deqing Zhou;D. Duan
诺如病毒（NoV）是全球病毒性胃肠炎爆发的主要原因，而牡蛎是诺如病毒污染和传播的最常见载体。 NoV 通过组织血型抗原 (HBGA) 与牡蛎组织特异性结合，从而促进病毒积累并增加病毒在牡蛎中的持久性。为了研究太平洋牡蛎中的 HBGA 与 GII.4 NoV 的相互作用，我们使用 ELISA 检查了 HBGA，并使用 P 粒子作为五个 GII.4 NoV 衣壳的模型，通过寡糖结合测定研究了结合模式。肠道和鳃中的 HBGA 表现出多态性。在肠道中，检测到 A 型（100%），而在鳃中检测到 Leb 型（91.67%）和 A 型（61.11%）。此外，我们发现季节性新型胃肠炎爆发与牡蛎肠道和鳃中检测到的特定 HBGA 没有显着相关性。在肠道中，我们发现菌株-2006b和菌株-96/96US与A型和H1型结合，但与Leb型仅弱结合；相反，Camberwell和Hunter菌株表现出与H1和Ley型的弱结合，并且Sakai菌株表现出与任何HBGA类型没有结合。在鳃中，96/96US 菌株和 2006b 菌株与 Leb 型结合，但与 H1 型结合较弱； Camberwell、Hunter 和 Sakai 菌株不与牡蛎 HBGA 结合。寡糖与 GII.4 NoV P 颗粒的结合分析显示，菌株 95/96US 和菌株 2006b 与 A、B、H1、Leb 和 Ley 型寡糖强烈结合，而菌株 Camberwell 和 Hunter 对 H1 型和 Ley 寡糖显示出较弱的结合能力，而菌株-Sakai 对 Leb 和 Ley 型寡糖显示出较弱的结合能力。我们的研究提供了新信息并加深了对牡蛎中 NoV 积累机制的理解。对多种NoV-组织相互作用的进一步研究可能有助于确定新的或改进的最小化污染策略，包括基于HBGA的附着抑制或净化。
Noroviruses (NoVs) are the main cause of viral gastroenteritis outbreaks worldwide, and oysters are the most common carriers of NoV contamination and transmission. NoVs bind specifically to oyster tissues through histo-blood group antigens (HBGAs), and this facilitates virus accumulation and increases virus persistence in oysters. To investigate the interaction of HBGAs in Pacific oysters with GII.4 NoV, we examined HBGAs with ELISAs and investigated binding patterns with oligosaccharide-binding assays using P particles as a model of five GII.4 NoV capsids. The HBGAs in the gut and gills exhibited polymorphisms. In the gut, type A was detected (100%), whereas type Leb (91.67%) and type A (61.11%) were both observed in the gills. Moreover, we found that seasonal NoV gastroenteritis outbreaks were not significantly associated with the specific HBGAs detected in the oyster gut and gills. In the gut, we found that strain-2006b and strain-96/96US bound to type A and H1 but only weakly bound to type Leb; in contrast, the Camberwell and Hunter strains exhibited weak binding to types H1 and Ley, and strain-Sakai exhibited no binding to any HBGA type. In the gills, strain-96/96US and strain-2006b bound to type Leb but only weakly bound to type H1; strains Camberwell, Hunter, and Sakai did not bind to oyster HBGAs. Assays for oligosaccharide binding to GII.4 NoV P particles showed that strain-95/96US and strain-2006b strongly bound to type A, B, H1, Leb, and Ley oligosaccharides, while strains Camberwell and Hunter showed weak binding ability to type H1 and Ley oligosaccharides and strain-Sakai showed weak binding ability to type Leb and Ley oligosaccharides. Our study presents new information and enhances understanding about the mechanism for NoV accumulation in oysters. Further studies of multiple NoV-tissue interactions might assist in identifying new or improved strategies for minimizing contamination, including HBGA-based attachment inhibition or depuration.
DOI：
10.1016/j.ijfoodmicro.2013.07.022
发表时间：
2013-09-02
影响因子：
5.4
作者：
Schaeffer J;Le Saux JC;Lora M;Atmar RL;Le Guyader FS
通讯作者：
Le Guyader FS