The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii.
The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii.
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DOI：
10.1038/s41438-021-00557-4
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发表时间：
2021-06-01
影响因子：
8.7
通讯作者：
Lin X
中科院分区：
文献类型：
--
作者：
Hou D;Li L;Ma T;Pei J;Zhao Z;Lu M;Wu A;Lin X
竹子以其笋可食、质地美观著称，具有相当的经济价值和观赏价值。竹类植物在传统开花植物中具有独特性，许多竹类植物在大范围同步开花后，会出现大面积死亡，严重影响竹林的生产力和应用。迄今为止，竹子开花特性的分子机制仍不清楚。本研究从绿竹中克隆了一个SOC 1样基因BoMADS 50。BoMADS 50在成熟叶片和花原基形成期（B）中高表达。Oldhamii开花和BoMADS 50的过量表达导致转基因水稻的早花。BoMADS 50在体内可与APETALA 1/FRUITFULL（AP 1/FUL）样蛋白（BoMADS 14 -1/2、BoMADS 15 -1/2）相互作用，BoMADS 14 -1可显著促进BoMADS 50的表达，进一步表明BoMADS 50与BoAP 1/FUL样蛋白在调控B中具有协同作用。Oldhamii开花我们还确定了BoMADS 50（BoMADS 50 -1/2/3/4）具有不同的核苷酸变异的四个额外的转录本。虽然蛋白质-CDS是多态性的，但它们具有与BoMADS 50相似的开花激活功能。随后的酵母单杂交和瞬时表达实验表明，BoMADS 50和BoMADS 50 -1都与自身的启动子片段和短营养期（SVP）样基因BoSVP结合，但只有BoMADS 50 -1能正向诱导它们的转录。因此，核苷酸变异可能赋予BoMADS 50 -1强的调节活性。因此，BoMADS 50和BoMADS 50 -1/2/3/4可能是B重要的开花正调控因子。oldhamii。BoMADS 50和BoMADS 50 -1的功能保守性和特异性可能与B的同步开花和零星开花特性有关。oldhamii。
Bamboo is known for its edible shoots and beautiful texture and has considerable economic and ornamental value. Unique among traditional flowering plants, many bamboo plants undergo extensive synchronized flowering followed by large-scale death, seriously affecting the productivity and application of bamboo forests. To date, the molecular mechanism of bamboo flowering characteristics has remained unknown. In this study, a SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1)-like gene, BoMADS50, was identified from Bambusa oldhamii. BoMADS50 was highly expressed in mature leaves and the floral primordium formation period during B. oldhamii flowering and overexpression of BoMADS50 caused early flowering in transgenic rice. Moreover, BoMADS50 could interact with APETALA1/FRUITFULL (AP1/FUL)-like proteins (BoMADS14-1/2, BoMADS15-1/2) in vivo, and the expression of BoMADS50 was significantly promoted by BoMADS14-1, further indicating a synergistic effect between BoMADS50 and BoAP1/FUL-like proteins in regulating B. oldhamii flowering. We also identified four additional transcripts of BoMADS50 (BoMADS50-1/2/3/4) with different nucleotide variations. Although the protein-CDS were polymorphic, they had flowering activation functions similar to those of BoMADS50. Yeast one-hybrid and transient expression assays subsequently showed that both BoMADS50 and BoMADS50-1 bind to the promoter fragment of itself and the SHORT VEGETATIVE PHASE (SVP)-like gene BoSVP, but only BoMADS50-1 can positively induce their transcription. Therefore, nucleotide variations likely endow BoMADS50-1 with strong regulatory activity. Thus, BoMADS50 and BoMADS50-1/2/3/4 are probably important positive flowering regulators in B. oldhamii. Moreover, the functional conservatism and specificity of BoMADS50 and BoMADS50-1 might be related to the synchronized and sporadic flowering characteristics of B. oldhamii.
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