Integrated analysis of gene expression and methylation profiles of novel pancreatic cancer cell lines with highly metastatic activity
Integrated analysis of gene expression and methylation profiles of novel pancreatic cancer cell lines with highly metastatic activity
复制标题
具有高转移活性的新型胰腺癌细胞系基因表达和甲基化谱的综合分析
DOI：
10.1007/s11427-018-9495-2
复制
发表时间：
2019-03
期刊：
影响因子：
--
通讯作者：
Zhao Yupei
中科院分区：
文献类型：
--
作者：
Yang Gang;Wang Huanyu;Feng Mengyu;You Lei;Zheng Lianfang;Zhang Taiping;Cong Lin;Zhao Yupei
Pancreatic cancer is one of the most lethal human malignancies, partly because of its propensity for metastasis. However, highly metastatic human pancreatic cancer cell lines suitable for studies of metastasis are currently lacking. Here we established two highly metastatic human pancreatic cancer cell lines, MIA PaCa-2 In8 and Panc-1 In8, by Matrigel induction assay. The cell lines were further characterized bothin vitroandin vivo. MIA PaCa-2 In8 and Panc-1 In8 cells demonstrated increased migration and invasion compared with their respective parental cells. Following injection into nude mice, MIA PaCa-2 In8 and Panc-1 In8 cells resulted in more pulmonary metastases compared with the parental cells. Furthermore, analyses of mRNA, long non-coding RNA, micro RNA, and methylation profiling revealed that these factors were aberrantly regulated in the highly metastatic cells, indicating that they probably affected metastasis. We thus established and characterized two highly metastatic human pancreatic cell lines that could be used as valuable tools for future investigations into the pathogenesis, metastasis, and potential treatment of human pancreatic cancer.
登录
查看更多内容
影响因子：
64.8
作者：
Notta F;Chan-Seng-Yue M;Lemire M;Li Y;Wilson GW;Connor AA;Denroche RE;Liang SB;Brown AM;Kim JC;Wang T;Simpson JT;Beck T;Borgida A;Buchner N;Chadwick D;Hafezi-Bakhtiari S;Dick JE;Heisler L;Hollingsworth MA;Ibrahimov E;Jang GH;Johns J;Jorgensen LG;Law C;Ludkovski O;Lungu I;Ng K;Pasternack D;Petersen GM;Shlush LI;Timms L;Tsao MS;Wilson JM;Yung CK;Zogopoulos G;Bartlett JM;Alexandrov LB;Real FX;Cleary SP;Roehrl MH;McPherson JD;Stein LD;Hudson TJ;Campbell PJ;Gallinger S
通讯作者：
Gallinger S
影响因子：
28.2
作者：
Karreth FA;Pandolfi PP
通讯作者：
Pandolfi PP
影响因子：
11.2
作者：
Goldstein JT;Berger AC;Shih J;Duke FF;Furst L;Kwiatkowski DJ;Cherniack AD;Meyerson M;Strathdee CA
通讯作者：
Strathdee CA
影响因子：
11.5
作者：
Hatano, Hiroko;Kudo, Yasusei;Takata, Takashi
通讯作者：
Takata, Takashi
影响因子：
2.9
作者：
Nishimori, H;Yasoshima, T;Hirata, K
通讯作者：
Hirata, K