Abnormal scar identification with spherical-nucleic-acid technology.
Abnormal scar identification with spherical-nucleic-acid technology.
复制标题
DOI：
10.1038/s41551-018-0218-x
复制
发表时间：
2018-04
影响因子：
28.1
通讯作者：
Xu C
中科院分区：
文献类型：
--
作者：
Yeo DC;Wiraja C;Paller AS;Mirkin CA;Xu C
The accurate diagnosis of scar type and severity relies on histopathology of biopsied tissue, which is invasive and time-consuming, causes discomfort and may exacerbate scarring. Here, we show that imaging nanoprobes for the live-cell detection of intracellular messenger RNA (mRNA) (also known as NanoFlares) enable measurements of the expression of connective tissue growth factor (CTGF) as a visual indicator of hypertrophic scars and keloids. During cell culture, NanoFlares enabled the distinction of hypertrophic and keloidal fibroblasts from normal fibroblasts, and the detection of changes in CTGF expression resulting from the regulatory effects of transforming growth factor-β (TGF-β) agonists and TGF-β antagonists. We also applied the NanoFlares topically to the skin of live mice and rabbits, and to ex vivo human skin models. Transepidermal penetration of the NanoFlares enabled the visual and spectroscopic quantification of underlying abnormal fibroblasts on the basis of CTGF mRNA expression. Our proof-of-concept studies of topically applied NanoFlare technology as a means of biopsy-free scar diagnosis may eventually inform therapeutic decisions on the basis of the mRNA-expression patterns of skin disorders.
登录
查看更多内容
影响因子：
3.6
作者：
PASYK, KA;ARGENTA, LC;HASSETT, C
通讯作者：
HASSETT, C
影响因子：
48
作者：
Lin, Tongxiang;Ambasudhan, Rajesh;Yuan, Xu;Li, Wenlin;Hilcove, Simon;Abujarour, Ramzey;Lin, Xiangyi;Hahm, Heung Sik;Hao, Ergeng;Hayek, Alberto;Ding, Sheng
通讯作者：
Ding, Sheng
影响因子：
15
作者：
Banga RJ;Meckes B;Narayan SP;Sprangers AJ;Nguyen ST;Mirkin CA
通讯作者：
Mirkin CA
DOI：
10.1073/pnas.1505951112
发表时间：
2015-05-05
影响因子：
11.1
作者：
Randeria, Pratik S.;Seeger, Mark A.;Paller, Amy S.
通讯作者：
Paller, Amy S.
影响因子：
23.9
作者：
Ichida JK;Blanchard J;Lam K;Son EY;Chung JE;Egli D;Loh KM;Carter AC;Di Giorgio FP;Koszka K;Huangfu D;Akutsu H;Liu DR;Rubin LL;Eggan K
通讯作者：
Eggan K