Tumor-Associated Macrophages and Regulatory T Cells Infiltration and the Clinical Outcome in Colorectal Cancer.
Tumor-Associated Macrophages and Regulatory T Cells Infiltration and the Clinical Outcome in Colorectal Cancer.
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DOI：
10.1007/s00005-017-0463-9
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发表时间：
2017-10
影响因子：
3.2
通讯作者：
Muc-Wierzgoń M
中科院分区：
文献类型：
--
作者：
Waniczek D;Lorenc Z;Śnietura M;Wesecki M;Kopec A;Muc-Wierzgoń M
该研究的目的是评估结直肠癌 (CRC) 患者中肿瘤相关巨噬细胞 (TAM) CD68+/iNOS− 和 Tregs CD8+/FoxP3+ 的浸润强度，作为无病生存 (DFS) 和总生存 (OS) 的预后因素。在这项回顾性研究中，从 89 名接受 CRC 切除术的患者（IIA 期、pT3N0M0 期以及 IIIB 和 IIIC 期、pT3N1-2M0）获取了组织样本。随访时观察到 45 名患者出现复发（10 名局部复发，35 名远处转移）。复发患者存在以下特征：诊断时平均年龄较高（p = 0.07）、淋巴结受累程度较高（p = 0.002）和临床疾病更晚期（p = 0.01）。采用组织学切片中TAM和Treg亚群鉴定的方法进行临床数据分析和免疫组化研究，旨在将其用于常规临床管理。 DSF 和 OS 都是该研究中评估的临床参数。肿瘤基质中 TAM 的强烈浸润与较短的 DFS (p = 0.005) 和 OS (p = 0.006) 相关。在肿瘤前沿观察到相反的趋势（p = 0.061）。肿瘤基质中 TAM 强烈浸润的患者组的复发和癌症相关死亡的相对风险高出两倍以上（分别为 RR 2.05，95% CI 1.33–3.14；p = 0.001 和 RR 2.08，95% CI 1.28–3.39；p = 0.003）。肿瘤基质中 Tregs 的强烈浸润与较短的 DFS 和 OS 相关 (p<<0.0001)。肿瘤基质中Treg细胞强烈浸润的患者组的复发和死亡的相对风险比浸润程度较低的患者高12倍以上（RR 12.3，95% CI 5.44–27.9；p < 0.0001和RR 12.5，95% CI 4.9–32.4；p < 0.0001，分别）。肿瘤基质中 TAM CD68+/iNOS− 和 Tregs CD8+/FoxP3+ 的浸润是负性预后因素，但它们之间呈正相关。 Tregs可能构成CRC患者的独立预后因素。
The aim of the study is the assessment of the intensity of the infiltration of tumor-associated macrophages (TAMs) CD68+/iNOS− and Tregs CD8+/FoxP3+ in colorectal cancer (CRC) patients as prognostic factors with respect to disease-free survival (DFS) and overall survival (OS). In this retrospective study, tissue samples were obtained from 89 patients undergoing resection for CRC (stage IIA, pT3N0M0 and stages IIIB and IIIC, pT3N1-2M0). Recurrence was observed in 45 patients at the time of the follow-up (10 local recurrences, 35 distant metastases). In patients with recurrence the following were present: a tendency to an older average age at the time of diagnosis (p = 0.07), higher nodal involvement (p = 0.002) and more advanced clinical disease (p = 0.01). The analysis of the clinical data and immunohistochemical studies were performed with the methodology of identification of TAM and Treg subsets in histological sections, with the aim to use it in routine clinical management. Both DSF and OS were the clinical parameters assessed in the study. The presence of intense infiltration of TAMs in the tumor stroma was related to shorter DFS (p = 0.005) and OS (p = 0.006). The opposite tendency was observed in the tumor front (p = 0.061). The relative risks of recurrence and cancer-related death were more than twice higher in the group of patients with intense infiltration of TAMs in the tumor stroma (RR 2.05, 95% CI 1.33–3.14; p = 0.001 and RR 2.08, 95% CI 1.28–3.39; p = 0.003, respectively). Intense infiltration of Tregs in the tumor stroma was related to shorter DFS and OS (p < 0.0001). The relative risks of recurrence and death in a group of patients with intense infiltration of Tregs in the tumor stroma were more than 12 times higher than in patients with less intense infiltration (RR 12.3, 95% CI 5.44–27.9; p < 0.0001 and RR 12.5, 95% CI 4.9–32.4; p < 0.0001, respectively). Infiltration of TAMs CD68+/iNOS− and Tregs CD8+/FoxP3+ in the tumor stroma are negative prognostic factors with a positive correlation between them. Tregs may constitute an independent prognostic factor in patients with CRC.
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影响因子：
1.2
作者：
Takenaka M;Seki N;Toh U;Hattori S;Kawahara A;Yamaguchi T;Koura K;Takahashi R;Otsuka H;Takahashi H;Iwakuma N;Nakagawa S;Fujii T;Sasada T;Yamaguchi R;Yano H;Shirouzu K;Kage M
通讯作者：
Kage M
影响因子：
45.3
作者：
Salama, Paul;Phillips, Michael;Iacopetta, Barry
通讯作者：
Iacopetta, Barry
影响因子：
3.7
作者：
Kim M;Grimmig T;Grimm M;Lazariotou M;Meier E;Rosenwald A;Tsaur I;Blaheta R;Heemann U;Germer CT;Waaga-Gasser AM;Gasser M
通讯作者：
Gasser M
DOI：
10.1084/jem.20050930
发表时间：
2006-04-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Kryczek I;Zou L;Rodriguez P;Zhu G;Wei S;Mottram P;Brumlik M;Cheng P;Curiel T;Myers L;Lackner A;Alvarez X;Ochoa A;Chen L;Zou W
通讯作者：
Zou W
影响因子：
11.5
作者：
Forssell, Johan;Oeberg, Ake;Palmqvist, Richard
通讯作者：
Palmqvist, Richard