Large-scale chromatin decondensation and recondensation regulated by transcription from a natural promoter.
Large-scale chromatin decondensation and recondensation regulated by transcription from a natural promoter.
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DOI：
10.1083/jcb.200011069
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发表时间：
2001-07-09
期刊：
影响因子：
--
通讯作者：
McNally JG
中科院分区：
文献类型：
--
作者：
Müller WG;Walker D;Hager GL;McNally JG
我们已经研究了转录和染色质结构之间的关系，使用串联阵列的小鼠乳腺肿瘤病毒（MMTV）启动子驱动的ras报告。该阵列在用绿色荧光蛋白（GFP）标记的糖皮质激素受体（GR）稳定转化的活细胞中被可视化为独特的荧光结构，GR在类固醇激素处理后定位于重复的MMTV元件。通过免疫荧光在阵列中还发现了两种不同的具有乙酰转移酶活性的类固醇受体辅激活因子（SRC 1和CBP）、染色质重塑因子（BRG 1）和两种转录因子（NFI和AP-2）。在加入激素后3 h内，通过GFP-GR或DNA荧光原位杂交（FISH）观察到的阵列不同程度地解密集，在最明显的情况下，从0.5 μm的斑点形成1-10 μm长的纤维。随后通过3-8小时的激素处理使阵列重新凝聚。解凝聚的程度与通过RNA FISH检测的由阵列产生的转录物的量成比例。去凝被两种不同的抑制聚合酶II的药物5，6-二氯-1-β-d-呋喃核糖基苯并咪唑（DRB）和α-鹅膏蕈碱阻断。这些观察结果证明了聚合酶在产生和维持去致密染色质中的作用。他们还支持纤维包装模型的高阶结构，并建议从一个天然的启动子转录可能会发生在更高的DNA包装密度比以前报道。
We have examined the relationship between transcription and chromatin structure using a tandem array of the mouse mammary tumor virus (MMTV) promoter driving a ras reporter. The array was visualized as a distinctive fluorescent structure in live cells stably transformed with a green fluorescent protein (GFP)-tagged glucocorticoid receptor (GR), which localizes to the repeated MMTV elements after steroid hormone treatment. Also found at the array by immunofluorescence were two different steroid receptor coactivators (SRC1 and CBP) with acetyltransferase activity, a chromatin remodeler (BRG1), and two transcription factors (NFI and AP-2). Within 3 h after hormone addition, arrays visualized by GFP-GR or DNA fluorescent in situ hybridization (FISH) decondensed to varying degrees, in the most pronounced cases from a ∼0.5-μm spot to form a fiber 1–10 μm long. Arrays later recondensed by 3–8 h of hormone treatment. The degree of decondensation was proportional to the amount of transcript produced by the array as detected by RNA FISH. Decondensation was blocked by two different drugs that inhibit polymerase II, 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole (DRB) and α-amanitin. These observations demonstrate a role for polymerase in producing and maintaining decondensed chromatin. They also support fiber-packing models of higher order structure and suggest that transcription from a natural promoter may occur at much higher DNA-packing densities than reported previously.
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影响因子：
64.5
作者：
LAMB, MM;DANEHOLT, B
通讯作者：
DANEHOLT, B
DOI：
10.1073/pnas.87.10.3977
发表时间：
1990-05-01
影响因子：
11.1
作者：
BRESNICK, EH;JOHN, S;HAGER, GL
通讯作者：
HAGER, GL
影响因子：
4.8
作者：
Kramer, PR;Fragoso, G;Sinden, RR
通讯作者：
Sinden, RR
影响因子：
64.8
作者：
Fryer, CJ;Archer, TK
通讯作者：
Archer, TK
DOI：
10.1073/pnas.49.4.544
发表时间：
1963-01-01
影响因子：
11.1
作者：
IZAWA, M;ALLFREY, VG;MIRSKY, AE
通讯作者：
MIRSKY, AE