Molecular evolution of type 2 porcine reproductive and respiratory syndrome viruses circulating in Vietnam from 2007 to 2015.
Molecular evolution of type 2 porcine reproductive and respiratory syndrome viruses circulating in Vietnam from 2007 to 2015.
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DOI：
10.1186/s12917-016-0885-3
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发表时间：
2016-11-17
影响因子：
2.6
通讯作者：
Le VP
中科院分区：
文献类型：
--
作者：
Do HQ;Trinh DT;Nguyen TL;Vu TT;Than DD;Van Lo T;Yeom M;Song D;Choe S;An DJ;Le VP
猪呼吸与生殖综合征（PRRS）病毒是越南养猪业中最具经济意义的病原体之一。ORF 5参与病毒粒子组装、病毒进入宿主细胞、病毒适应宿主免疫应答等多种功能过程，已被广泛应用于分子进化和生殖发生学研究。了解PRRSV野毒株的分子进化可能有助于越南的PRRS控制。系统发育分析表明，所有菌株均属于8.7和5.1亚系。核苷酸同源性为84.5-100%，氨基酸同源性为82- 100%。此外，结果揭示了2个亚谱系组之间的核苷酸和氨基酸同一性的差异。N-糖基化预测确定了7个潜在的N-糖基化位点和11种糖型。GP 5序列分析显示，7个位点处于正选择压力下，25个位点处于负选择压力下。ORF 5序列的系统发育分析表明，越南的PRRSV的多样性。此外，N-糖基化位点和位置的选择压力下的变化进行了论证。该研究扩展了有关越南PRRS遗传多样性和进化的现有知识，并为越南PRRS疫苗开发的有效策略提供帮助。本文的在线版本（doi：10.1186/s12917-016-0885-3）包含补充材料，可供授权用户使用。
Porcine respiratory and reproductive syndrome (PRRS) virus is one of the most economically significant pathogens in the Vietnamese swine industry. ORF5, which participates in many functional processes, including virion assembly, entry of the virus into the host cell, and viral adaptation to the host immune response, has been widely used in molecular evolution and phylogeny studies. Knowing of molecular evolution of PRRSV fields strains might contribute to PRRS control in Vietnam. The results showed that phylogenetic analysis indicated that all strains belonged to sub-lineages 8.7 and 5.1. The nucleotide and amino acid identities between strains were 84.5–100% and 82–100%, respectively. Furthermore, the results revealed differences in nucleotide and amino acid identities between the 2 sub-lineage groups. N-glycosylation prediction identified 7 potential N-glycosylation sites and 11 glycotypes. Analyses of the GP5 sequences, revealed 7 sites under positive selective pressure and 25 under negative selective pressure. Phylogenetic analysis based on ORF5 sequence indicated the diversity of PRRSV in Vietnam. Furthermore, the variance of N-glycosylation sites and position under selective pressure were demonstrated. This study expands existing knowledge on the genetic diversity and evolution of PRRSV in Vietnam and assists the effective strategies for PRRS vaccine development in Vietnam. The online version of this article (doi:10.1186/s12917-016-0885-3) contains supplementary material, which is available to authorized users.
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