Visualizing transfer of microbial biomolecules by outer membrane vesicles in microbe-host-communication in vivo.
Visualizing transfer of microbial biomolecules by outer membrane vesicles in microbe-host-communication in vivo.
复制标题
DOI：
10.1002/jev2.12159
复制
发表时间：
2021-10
影响因子：
16
通讯作者：
Momma S
中科院分区：
文献类型：
--
作者：
Bittel M;Reichert P;Sarfati I;Dressel A;Leikam S;Uderhardt S;Stolzer I;Phu TA;Ng M;Vu NK;Tenzer S;Distler U;Wirtz S;Rothhammer V;Neurath MF;Raffai RL;Günther C;Momma S
肠道微生物群影响哺乳动物宿主的生理健康和疾病，不仅在肠道局部，而且在没有与细菌直接接触的器官如肝脏和大脑中。由微生物释放的细胞外囊泡（EV）或外膜囊泡（OMV）越来越多地被认为是王国间通信的生物穿梭系统的潜在作用。然而，在体内通过OMV将功能性生物分子从肠道微生物群转移到个体宿主细胞的生理学相关证据很少。通过将经工程改造以表达Cre-重组酶的大肠杆菌（E. coli Cre）引入具有Rosa26.tdTomato-报告基因背景的小鼠中，我们利用Cre-LoxP系统来报告细菌OMV向体内受体细胞的转移。用大肠杆菌Cre定殖这些小鼠的肠，导致Cre重组酶诱导荧光报告基因在沿着肠上皮的细胞中表达，包括肠干细胞以及粘膜免疫细胞如巨噬细胞。此外，即使远远超出肠道，细菌来源的Cre在广泛的宿主组织中诱导扩展标记基因表达，包括心脏，肝脏，肾脏，脾脏和大脑。总之，我们的研究结果提供了一种方法和原理证明，OMV可以作为一种生物穿梭系统，用于细菌和哺乳动物宿主细胞之间的功能性生物分子的水平转移。
The intestinal microbiota influences mammalian host physiology in health and disease locally in the gut but also in organs devoid of direct contact with bacteria such as the liver and brain. Extracellular vesicles (EVs) or outer membrane vesicles (OMVs) released by microbes are increasingly recognized for their potential role as biological shuttle systems for inter‐kingdom communication. However, physiologically relevant evidence for the transfer of functional biomolecules from the intestinal microbiota to individual host cells by OMVs in vivo is scarce. By introducing Escherichia coli engineered to express Cre‐recombinase (E. coli Cre) into mice with a Rosa26.tdTomato‐reporter background, we leveraged the Cre‐LoxP system to report the transfer of bacterial OMVs to recipient cells in vivo. Colonizing the intestine of these mice with E. coli Cre, resulted in Cre‐recombinase induced fluorescent reporter gene‐expression in cells along the intestinal epithelium, including intestinal stem cells as well as mucosal immune cells such as macrophages. Furthermore, even far beyond the gut, bacterial‐derived Cre induced extended marker gene expression in a wide range of host tissues, including the heart, liver, kidney, spleen, and brain. Together, our findings provide a method and proof of principle that OMVs can serve as a biological shuttle system for the horizontal transfer of functional biomolecules between bacteria and mammalian host cells.
登录
查看更多内容
影响因子：
15.5
作者：
Durant, Lydia;Stentz, Regis;Knight, Stella C.
通讯作者：
Knight, Stella C.
影响因子：
3.7
作者：
Green ER;Mecsas J
通讯作者：
Mecsas J
影响因子：
15.9
作者：
Becker, C;Wirtz, S;Neurath, MF
通讯作者：
Neurath, MF
影响因子：
3.7
作者：
Duong, Phat;Chung, Allen;Raffai, Robert L.
通讯作者：
Raffai, Robert L.
DOI：
10.1126/science.aad9948
发表时间：
2016-05-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chu H;Khosravi A;Kusumawardhani IP;Kwon AH;Vasconcelos AC;Cunha LD;Mayer AE;Shen Y;Wu WL;Kambal A;Targan SR;Xavier RJ;Ernst PB;Green DR;McGovern DP;Virgin HW;Mazmanian SK
通讯作者：
Mazmanian SK