In situ mechanical properties of the chondrocyte cytoplasm and nucleus.
In situ mechanical properties of the chondrocyte cytoplasm and nucleus.
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DOI：
10.1016/j.jbiomech.2009.01.024
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发表时间：
2009-05-11
影响因子：
2.4
通讯作者：
Athanasiou, Kyriacos A.
中科院分区：
文献类型：
--
作者：
Ofek, Gidon;Natoli, Roman M.;Athanasiou, Kyriacos A.
细胞核感受机械力的方式对于理解机械力传导具有重要意义。核材料性质的知识，特别是它们与本体细胞性质的关系，可以帮助确定细胞核是否直接经历机械负荷，或者是否是继发于细胞膜变形的信号转导导致基因表达改变。先前的工作测量核材料的性质，使用微管抽吸表明，核是实质上比散装细胞更硬，而最近的工作与无限制压缩的单个软骨细胞显示出近一对一的细胞和核应变之间的相关性。在这项研究中，一个线弹性有限元模型的细胞与核夹杂被用来模拟无侧限压缩数据。对于细胞质和细胞核的泊松比的几种组合，细胞质和细胞核的刚度在1至7 kPa之间变化。结果发现，当细胞质与细胞核的刚度比为1.4，细胞质和细胞核都被模拟为不可压缩时，实验数据拟合得最好。细胞质与细胞核的刚度比显著低于先前报道的孤立细胞核。这些结果表明，细胞核在原位的力学行为可能与分离时不同。
The way in which the nucleus experiences mechanical forces has important implications for understanding mechanotransduction. Knowledge of nuclear material properties and, specifically, their relationship to the properties of the bulk cell can help determine if the nucleus directly experiences mechanical load, or if it is signal transduction secondary to cell membrane deformation that leads to altered gene expression. Prior work measuring nuclear material properties using micropipette aspiration suggests that the nucleus is substantially stiffer than the bulk cell, whereas recent work with unconfined compression of single chondrocytes showed a nearly one-to-one correlation between cellular and nuclear strains. In this study, a linearly elastic finite element model of the cell with a nuclear inclusion was used to simulate the unconfined compression data. Cytoplasmic and nuclear stiffnesses were varied from 1 to 7 kPa for several combinations of cytoplasmic and nuclear Poisson’s ratios. It was found that the experimental data were best fit when the ratio of cytoplasmic to nuclear stiffness was 1.4, and both cytoplasm and nucleus were modeled as incompressible. The cytoplasmic to nuclear stiffness ratio is significantly lower than prior reports for isolated nuclei. These results suggest the nucleus may behave mechanically different in situ than when isolated.
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影响因子：
2.4
作者：
Lee, DA;Knight, MM;Bader, DL
通讯作者：
Bader, DL
DOI：
10.1073/pnas.94.3.849
发表时间：
1997-02-04
影响因子：
11.1
作者：
Maniotis, AJ;Chen, CS;Ingber, DE
通讯作者：
Ingber, DE
影响因子：
2.4
作者：
Koay, Eugene J.;Ofek, Gidon;Athanasiou, Kyriacos A.
通讯作者：
Athanasiou, Kyriacos A.
影响因子：
2.4
作者：
Trickey, WR;Baaijens, FPT;Guilak, F
通讯作者：
Guilak, F
影响因子：
3.6
作者：
Eleswarapu, Sriram V.;Leipzig, Nic D.;Athanasiou, Kyriacos A.
通讯作者：
Athanasiou, Kyriacos A.