In vitro induction of cartilage-specific macromolecules by a bone extract
In vitro induction of cartilage-specific macromolecules by a bone extract
复制标题
通过骨提取物体外诱导软骨特异性大分子
作者：
S. Seyedin;A. Thompson;D. Rosen;K. Piez
已经开发出体外系统来研究软骨形成的发生。当用牛骨基质提取物进行悬浮培养时，胚胎大鼠肌肉间充质细胞会合成软骨特异性蛋白多糖和 II 型胶原蛋白。通过酶联免疫吸附测定抑制技术测定了这两种大分子的合成。当在坚固的琼脂糖中培养并进行异染基质染色时，处理的细胞的形态变化证明了软骨形成的进一步证据。即使使用粗骨基质提取物，该测定在微克水平上也很敏感，并且在 2-3 天内观察到与对照相比软骨大分子的显着差异。在体内，相同的提取物首先诱导软骨，然后诱导骨。
An in vitro system has been developed to study the onset of chondrogenesis. Embryonic rat muscle mesenchymal cells, when treated in suspension culture with an extract of bovine bone matrix, synthesized cartilage-specific proteoglycan and type II collagen. The synthesis of these two macromolecules was assayed by the enzyme-linked immunosorbent assay inhibition technique. Further evidence of chondrogenesis was demonstrated by morphological changes of treated cells when cultured in firm agarose and stained for metachromatic matrix. Even with crude bone matrix extracts, the assay was sensitive at the microgram level and significant differences in cartilage macromolecules compared with controls were observed in 2-3 d. In vivo the same extract induced first cartilage and then bone.
影响因子：
2.9
作者：
Farley,JR;Baylink,DJ
通讯作者：
Baylink,DJ
骨基质上生长的骨骼肌的软骨分化分析。
影响因子：
2.7
作者：
Nathanson,MA;Hay,ED
通讯作者：
Hay,ED