β-Cryptoxanthin Attenuates Cigarette-Smoke-Induced Lung Lesions in the Absence of Carotenoid Cleavage Enzymes (BCO1/BCO2) in Mice.
β-Cryptoxanthin Attenuates Cigarette-Smoke-Induced Lung Lesions in the Absence of Carotenoid Cleavage Enzymes (BCO1/BCO2) in Mice.
复制标题
DOI：
10.3390/molecules28031383
复制
发表时间：
2023-02-01
期刊：
影响因子：
4.6
通讯作者：
Wang, Xiang-Dong
中科院分区：
文献类型：
--
作者：
Chiaverelli, Rachel A. A.;Hu, Kang-Quan;Liu, Chun;Lim, Ji Ye;Daniels, Michael S. S.;Xia, Hui;Mein, Jonathan;von Lintig, Johannes;Wang, Xiang-Dong
关键词：
膳食中大量摄入 β-隐黄质（BCX，一种含氧维生素原 A 类胡萝卜素）与吸烟者患肺部疾病的风险较低有关。 BCX 可以被 β-胡萝卜素-15,15'-加氧酶 (BCO1) 和 β-胡萝卜素-9',10'-加氧酶 (BCO2) 裂解，产生视黄醇和 apo-10'-类胡萝卜素。我们研究了 BCX 是否对香烟烟雾 (CS) 引起的肺损伤具有保护作用，该作用依赖于或独立于 BCO1/BCO2 及其代谢物。 BCO1−/−/BCO2−/− 双敲除小鼠 (DKO) 和野生型 (WT) 同窝小鼠均补充 BCX 14 天，然后再暴露于 CS 14 天。与未暴露的同窝小鼠相比，无论基因型如何，CS暴露均显着诱导小鼠肺组织中的巨噬细胞和中性粒细胞浸润。无论性别如何，BCX 治疗均显着抑制 WT 和 DKO 小鼠中 CS 诱导的炎症细胞浸润、支气管上皮增生和肺泡空腔扩大。 BCX 的保护作用与 IL-6、TNF-α 以及基质金属蛋白酶-2 和 -9 的表达降低有关。 BCX 治疗导致 DKO 小鼠肝脏 BCX 水平显着增加，但 WT 小鼠则不然，后者肝脏视黄醇浓度显着增加。在任何组中均未检测到apo-10'-类胡萝卜素。体外 BCX，在相当剂量的 3-OH-β-apo-10'-胡萝卜醛下，可有效抑制人支气管上皮细胞系中脂多糖诱导的炎症反应。这些数据表明，在没有类胡萝卜素裂解酶的情况下，BCX 可以作为针对 CS 引起的肺部病变的有效保护剂。
High dietary intake of β-cryptoxanthin (BCX, an oxygenated provitamin A carotenoid) is associated with a lower risk of lung disease in smokers. BCX can be cleaved by β-carotene-15,15′-oxygenase (BCO1) and β-carotene-9′,10′-oxygenase (BCO2) to produce retinol and apo-10′-carotenoids. We investigated whether BCX has protective effects against cigarette smoke (CS)-induced lung injury, dependent or independent of BCO1/BCO2 and their metabolites. Both BCO1−/−/BCO2−/− double knockout mice (DKO) and wild type (WT) littermates were supplemented with BCX 14 days and then exposed to CS for an additional 14 days. CS exposure significantly induced macrophage and neutrophil infiltration in the lung tissues of mice, regardless of genotypes, compared to the non-exposed littermates. BCX treatment significantly inhibited CS-induced inflammatory cell infiltration, hyperplasia in the bronchial epithelium, and enlarged alveolar airspaces in both WT and DKO mice, regardless of sex. The protective effects of BCX were associated with lower expression of IL-6, TNF-α, and matrix metalloproteinases-2 and -9. BCX treatment led to a significant increase in hepatic BCX levels in DKO mice, but not in WT mice, which had significant increase in hepatic retinol concentration. No apo-10′-carotenoids were detected in any of the groups. In vitro BCX, at comparable doses of 3-OH-β-apo-10′-carotenal, was effective at inhibiting the lipopolysaccharide-induced inflammatory response in a human bronchial epithelial cell line. These data indicate that BCX can serve as an effective protective agent against CS-induced lung lesions in the absence of carotenoid cleavage enzymes.
登录
查看更多内容
DOI：
10.1158/1940-6207.capr-10-0384
发表时间：
2011-08
期刊：
Cancer prevention research (Philadelphia, Pa.)
影响因子：
--
作者：
Liu C;Bronson RT;Russell RM;Wang XD
通讯作者：
Wang XD
DOI：
10.1165/rcmb.2011-0353oc
发表时间：
2012-07-01
影响因子：
6.4
作者：
Herfs, Michael;Hubert, Pascale;Delvenne, Philippe
通讯作者：
Delvenne, Philippe
影响因子：
4.2
作者：
Ford, Nikki A.;Clinton, Steven K.;Erdman, John W., Jr.
通讯作者：
Erdman, John W., Jr.
影响因子：
4.8
作者：
Amengual, Jaume;Widjaja-Adhi, M. Airanthi K.;von Lintig, Johannes
通讯作者：
von Lintig, Johannes
影响因子：
4.8
作者：
Kiefer, C;Hessel, S;von Lintig, J
通讯作者：
von Lintig, J