Immunocytochemical localization of hepatic fatty acid binding protien in the rat intestine: Effect of fasting
Immunocytochemical localization of hepatic fatty acid binding protien in the rat intestine: Effect of fasting
复制标题
大鼠肠道中肝脂肪酸结合蛋白的免疫细胞化学定位：禁食的影响
DOI：
--
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
H. Kondo
中科院分区：
文献类型：
--
作者：
S. Iseki;M. Hitomi;T. Ono;H. Kondo
Localization of hepatic fatty acid binding protein (h‐FABP) in the small and large intestines of rats was studied by light and electron microscopic histochemistry using the peroxidase‐antiperoxidase (PAP) method. In the small intestine of rats fed ad libitum, an intense FABP immunoreactivity was confined to the absorptive epithelial cells of the villi, but not of the crypts. The lowest margin of the immunoreactive cell sheet was closer to the crypts in the proximal than in the distal portions of the small intestine. In the large intestine, FABP immunoreactivity was present in the surface epithelial cells, with higher intesity in the proximal than in the distal portions of the intestine. After fasting rats for two days, many crypt cells exhibited intense immunoreactivity for h‐FABP, resulting in an extension of the lowest margin of the immunoreactive cell sheet deep into the crypts. Such expansion of the immunoreactive cell population was reversed by refeeding the animals. With regard to the intracellular localization of immunoreactivity in the jejunum, the basolateral portion of the cytoplasm exhibited a more intense immunoreaction than the apical portion in the majority of immunoreactive cells lining the villi, whether the animals were fed or fasted. The immunoreactive products appeared in the cytoplasmic matix without association with any subcellular structures. Although clusters of cells located in the villous tips exhibited evenly dense immunostaining throughout the entire cytoplasm, no increasing gradient in the intensity of the immunoreactivity from the base to the apex of the cytoplasm was seen in any epithelial cells. The present findings imply that h‐FABP could be involved in the uptake and metabolism of plasma‐derived as well as dietary‐free fatty acids by the intestinal epithelial cells.
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编码大鼠肠道前载脂蛋白 AIV 信使核糖核酸部分的互补脱氧核糖核酸的克隆。
影响因子：
2.9
作者：
Gordon,JI;Smith,DP;Alpers,DH;Strauss,AW
通讯作者：
Strauss,AW
大鼠肝脏和肠道中两种不同脂肪酸结合蛋白生物合成的调节。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bass,NM;Manning,JA;Ockner,RK;Gordon,JI;Seetharam,S;Alpers,DH
通讯作者：
Alpers,DH
DOI：
10.1016/s0021-9258(18)89501-1
发表时间：
1985-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J I Gordon;N. Elshourbagy;J. B. Lowe;W S Liao;D. Alpers;J. M. Taylor
通讯作者：
J I Gordon;N. Elshourbagy;J. B. Lowe;W S Liao;D. Alpers;J. M. Taylor
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ockner,RK;Manning,JA;Kane,JP
通讯作者：
Kane,JP