Receptor-rich intracellular membrane vesicles transporting asialotransferrin and insulin in liver
Receptor-rich intracellular membrane vesicles transporting asialotransferrin and insulin in liver
复制标题
肝脏中富含受体的细胞内膜囊泡转运脱唾液酸转铁蛋白和胰岛素
作者：
M. Debanne;W. H. Evans;N. Flint;E. Regoeczi
广泛的受体位于肝细胞质膜的血窦面1，2。许多与细胞表面受体结合的循环配体沿沿着两条途径被修饰。去唾液酸糖蛋白3从质膜转移到溶酶体并降解，而免疫球蛋白A4和胆汁酸5则通过肝细胞内部转运并释放到胆汁中。无唾液酸转铁蛋白3型（参考文献6）遵循称为胞浆融合的进一步途径7。在与脱唾液酸糖蛋白受体结合后8，脱唾液酸转铁蛋白被内吞，然后返回血液，其碳水化合物侧链的一部分被再唾液酸化9。我们现在描述在肝脏中的细胞内的无唾液酸转铁蛋白封闭囊泡（diacytosomes）的属性，并表明它们不同于高尔基体，溶酶体和质膜部分。此外，我们表明，脱唾液酸糖蛋白结合位点位于双酰基细胞体的细胞质（外）表面上。
A wide range of receptors are located at the blood sinusoidal aspect of the hepatocyte plasma membrane1,2. Many circulating ligands that bind to receptors on the cell surfaces are interiorized along two pathways. Asialoglycoproteins3 are transferred from the plasma membrane to lysosomes and degraded, whereas immunoglobulin A4 and bile acids5 are transported across the hepatocyte interior and released into bile. Asialotransferrin type 3 (ref. 6) follows a further pathway termed diacytosis7. After binding to the asialoglycoprotein receptor8, asialotransferrin is endocytosed and then returned to blood with a proportion of its carbohydrate side chains resialylated9. We now describe in liver the properties of intracellular asialotransferrin-enclosing vesicles (diacytosomes) and show that they differ from Golgi, lysosome and plasma membrane fractions. Furthermore, we show that the asialoglycoprotein binding sites are located on the cytoplasmic (outer) surface of diacytosomes.
大鼠肝脏中的磷酸甘露糖基酶受体。
DOI：
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发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Fischer,HD;Gonzalez-Noriega,A;Sly,WS;Morré,DJ
通讯作者：
Morré,DJ
影响因子：
56.9
作者：
ROTHMAN, JE
通讯作者：
ROTHMAN, JE