Human nonmuscle myosin heavy chain mRNA: generation of diversity through alternative polyadenylylation.
Human nonmuscle myosin heavy chain mRNA: generation of diversity through alternative polyadenylylation.
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人非肌肉肌球蛋白重链 mRNA：通过选择性聚腺苷酸化产生多样性。
DOI：
10.1073/pnas.87.3.1164
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发表时间：
1990
影响因子：
11.1
通讯作者：
Leinwand,LA
中科院分区：
文献类型：
--
作者：
Saez,CG;Myers,JC;Shows,TB;Leinwand,LA
肌球蛋白是一种普遍存在的真核收缩蛋白，它产生的力负责肌肉收缩和胞质分裂等多种细胞运动。虽然已经有大量的研究肌节肌球蛋白重链（MHC）基因，没有分子克隆已被报道编码哺乳动物非肌肉MHC。这项研究提出了人非肌肉MHC的分子遗传学特征，在成纤维细胞，内皮细胞和巨噬细胞中表达。人非肌肉MHC氨基酸与肌节MHC弱同源（33%），但与平滑肌MHC约72%相同。与脊椎动物肌节MHC通过多基因家族成员的表达产生多样性相反，在非肌肉MHC基因中使用替代的聚腺苷酸化位点来产生编码相同蛋白质的多个转录本。我们已经把这个基因定位在22号染色体上。因此，它与人类14号和17号染色体上的肌节MHC基因簇中的任何一个都不连锁。
Myosin is a ubiquitous eukaryotic contractile protein that generates the force responsible for such diverse cellular movements as muscle contraction and cytokinesis. Although there have been numerous studies of sarcomeric myosin heavy chain (MHC) genes, no molecular clones have been reported that encode mammalian nonmuscle MHC. This study presents the molecular genetic characterization of a human nonmuscle MHC that is expressed in fibroblasts, endothelial cells, and macrophages. Human nonmuscle MHC amino acids are weakly homologous (33%) to sarcomeric MHC but are approximately 72% identical to smooth muscle MHC. In contrast to vertebrate sarcomeric MHCs, which generate diversity through the expression of members of a multigene family, an alternative polyadenylylation site is used in the nonmuscle MHC gene to generate multiple transcripts that encode the same protein. We have mapped this gene to chromosome 22. It is thus unlinked to either of the sarcomeric MHC gene clusters on human chromosomes 14 and 17.
人类细胞质肌动蛋白由多基因家族编码
影响因子：
5.3
作者：
J. Engel;P. Gunning;L. Kedes
通讯作者：
L. Kedes