Spectroscopic verification of zinc absorption and distribution in the desert plant Prosopis juliflora-velutina (velvet mesquite) treated with ZnO nanoparticles.
Spectroscopic verification of zinc absorption and distribution in the desert plant Prosopis juliflora-velutina (velvet mesquite) treated with ZnO nanoparticles.
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DOI：
10.1016/j.cej.2010.12.021
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发表时间：
2011-06-01
期刊：
影响因子：
--
通讯作者：
Gardea-Torresdey JL
中科院分区：
文献类型：
--
作者：
Hernandez-Viezcas JA;Castillo-Michel H;Servin AD;Peralta-Videa JR;Gardea-Torresdey JL
金属纳米颗粒（NPs）对生物系统，特别是植物的影响仍然没有得到很好的理解。本研究的目的是确定氧化锌（ZnO）纳米颗粒在天鹅绒牧豆（牧豆）的影响。在具有浓度从500至4000 mg L-1变化的ZnO NP（10 nm）的水培中使豆科灌木幼苗生长15天。采用电感耦合等离子体发射光谱法（ICP-OES）测定了根、茎和叶中锌的含量。通过过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APOX）的比活性检测植物胁迫;通过X射线吸收光谱（XAS）和微区X射线荧光（μXRF）分别测定ZnO纳米颗粒的生物转化和Zn在组织中的分布。ICP-OES结果显示，在2000 mg ZnO NPs L−1时，组织中的Zn浓度（根、茎和叶中分别为2102 ± 87、1135 ± 56和628 ± 130 mg kg−1 d wt）。胁迫试验表明，ZnO纳米颗粒增加了根，茎，叶中的CAT，而APOX只增加茎和叶。XANES光谱表明，ZnO纳米颗粒不存在于豆科植物组织中，而Zn被发现为Zn（II），类似于Zn（NO3）2的光谱。μXRF分析证实了ZnO NP处理植物的根和叶的维管系统中存在Zn。
The impact of metal nanoparticles (NPs) on biological systems, especially plants, is still not well understood. The aim of this research was to determine the effects of zinc oxide (ZnO) NPs in velvet mesquite (Prosopis juliflora-velutina). Mesquite seedlings were grown for 15 days in hydroponics with ZnO NPs (10 nm) at concentrations varying from 500 to 4000 mg L−1. Zinc concentrations in roots, stems and leaves were determined by inductively coupled plasma optical emission spectroscopy (ICP-OES). Plant stress was examined by the specific activity of catalase (CAT) and ascorbate peroxidase (APOX); while the biotransformation of ZnO NPs and Zn distribution in tissues was determined by X-ray absorption spectroscopy (XAS) and micro X-ray fluorescence (μXRF), respectively. ICP-OES results showed that Zn concentrations in tissues (2102 ± 87, 1135 ± 56, and 628 ± 130 mg kg−1 d wt in roots, stems, and leaves, respectively) were found at 2000 mg ZnO NPs L−1. Stress tests showed that ZnO NPs increased CAT in roots, stems, and leaves, while APOX increased only in stems and leaves. XANES spectra demonstrated that ZnO NPs were not present in mesquite tissues, while Zn was found as Zn(II), resembling the spectra of Zn(NO3)2. The μXRF analysis confirmed the presence of Zn in the vascular system of roots and leaves in ZnO NP treated plants.
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