The Study of Protein-DNA Interactions in CD4+ T-Cells Using ChIPmentation.
The Study of Protein-DNA Interactions in CD4+ T-Cells Using ChIPmentation.
复制标题
使用 ChIPmentation 研究 CD4 T 细胞中的蛋白质-DNA 相互作用。
DOI：
10.1007/978-1-0716-1311-5_17
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Hertweck A
中科院分区：
文献类型：
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作者：
Hertweck A
染色质免疫沉淀（ChIP）结合高通量测序（ChIP-seq）是一种非常宝贵的方法，可以在基因组中富集组蛋白修饰和转录因子结合位点。然而，标准的ChIP-seq方案需要大量的细胞（>107）作为起始材料，这对于罕见的免疫群体通常是不可能获得的。在这里，我们描述了一个精简的ChIP协议优化的小细胞数与转座子标记介导的测序文库制备（ChIPmentation），允许分析的样品低至105个细胞。
Chromatin immunoprecipitation (ChIP) coupled with high-throughput sequencing (ChIP-seq) is an invaluable method to profile of enrichment of histone modifications and transcription factor binding sites across the genome. However, standard ChIP-seq protocols require large numbers of cells (>107) as starting material, which are often impossible to obtain for rare immune populations. Here we describe a streamlined ChIP protocol optimised for small cell numbers in conjunction with transposon-tagging mediated sequencing library preparation (ChIPmentation) which allows the analysis of samples of as low as 105cells.
影响因子：
48
作者：
Buenrostro, Jason D.;Giresi, Paul G.;Zaba, Lisa C.;Chang, Howard Y.;Greenleaf, William J.
通讯作者：
Greenleaf, William J.