Human adipose-derived cells can serve as a single-cell source for the in vitro cultivation of vascularized bone grafts.
Human adipose-derived cells can serve as a single-cell source for the in vitro cultivation of vascularized bone grafts.
复制标题
DOI：
10.1002/term.1564
复制
发表时间：
2014-08
影响因子：
3.3
通讯作者：
Vunjak-Novakovic G
中科院分区：
文献类型：
--
作者：
Correia C;Grayson W;Eton R;Gimble JM;Sousa RA;Reis RL;Vunjak-Novakovic G
骨科手术通常需要骨移植来纠正因先天性缺陷、手术或创伤而导致的巨大缺陷。组织工程学在骨移植方面取得了很大的进步。然而，这些移植物缺乏对其生存和功能至关重要的血管成分。从临床角度来看，从患者获取的单个细胞开始设计带血管的骨移植将是理想的。为此，我们探索了人脂肪来源的间充质干细胞(HASC)作为一种单一细胞来源的潜能，用于成骨和内皮分化，以及在同一支架内组装骨和血管间室。HASC被包裹在纤维蛋白水凝胶中作为血管形成的诱导材料，与多孔丝素海绵结合以支持成骨，并接受生长因子的连续应用。通过改变时空线索评估了三种策略：1)在血管生成之前诱导成骨，2)在成骨之前诱导血管生成，或3)同时诱导成骨和血管生成。培养5周后，骨基质蛋白沉积、碱性磷酸酶活性和钙沉积，CD31、von Willebrand因子等内皮细胞表面标志的血管网络形成，形态计量学分析证实类骨样组织发育。两个组织室最强劲的发育是通过依次诱导成骨和诱导血管生成来实现的。综上所述，收集的数据有力地支持了HASC作为血管化骨组织形成的单一细胞来源的用途。
Orthopedic surgery often requires bone grafts to correct large defects resulting from congenital defects, surgery or trauma. Great improvements have been made in tissue engineering of bone grafts. However, these grafts lack the vascularized component that is critical for their survival and function. From a clinical perspective, it would be ideal to engineer vascularized bone grafts starting from one single cell harvest obtained from the patient. To this end, we explored the potential of human adipose derived mesenchymal stem cells (hASC) as a single cell source for osteogenic and endothelial differentiation and the assembly of bone and vascular compartments within the same scaffold. hASC were encapsulated in fibrin hydrogel as a angioinductive material for vascular formation, combined with a porous silk fibroin sponge to support osteogenesis, and subjected to sequential application of growth factors. Three strategies were evaluated by changing spatio-temporal cues: 1) induction of osteogenesis prior to vasculogenesis, 2) induction of vasculogenesis prior to osteogenesis, or 3) simultaneous induction of osteogenesis and vasculogenesis. By 5 weeks of culture, bone-like tissue development was evidenced by the deposition of bone matrix proteins, alkaline phosphatase activity and calcium deposition, along with the formation of vascular networks evidenced by endothelial cell surface markers, such as CD31 and von Willebrand factor, and morphometric analysis. Most robust development of the two tissue compartments was achieved by sequential induction of osteogenesis followed by the induction of vasculogenesis. Taken together, the collected data strongly support the utility of hASC as a single cell source for the formation of vascularized bone tissue.
登录
查看更多内容
影响因子：
4.5
作者：
Ghazalpour A;Bennett B;Petyuk VA;Orozco L;Hagopian R;Mungrue IN;Farber CR;Sinsheimer J;Kang HM;Furlotte N;Park CC;Wen PZ;Brewer H;Weitz K;Camp DG 2nd;Pan C;Yordanova R;Neuhaus I;Tilford C;Siemers N;Gargalovic P;Eskin E;Kirchgessner T;Smith DJ;Smith RD;Lusis AJ
通讯作者：
Lusis AJ
影响因子：
5.2
作者：
Mitchell, James B.;McIntosh, Kevin;Gimble, Jeffrey M.
通讯作者：
Gimble, Jeffrey M.
影响因子：
5.2
作者：
McIntosh, Kevin;Zvonic, Sanjin;Gimble, Jeffrey M.
通讯作者：
Gimble, Jeffrey M.
影响因子：
37.8
作者：
Rehman, J;Traktuev, D;March, KL
通讯作者：
March, KL
DOI：
10.1007/s10856-005-4709-4
发表时间：
2005-12-01
影响因子：
3.7
作者：
Malafaya, PB;Pedro, AJ;Reis, RL
通讯作者：
Reis, RL