Flying through the drosophila cytoskeletal genome.
Flying through the drosophila cytoskeletal genome.
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DOI：
10.1083/jcb.150.2.f63
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发表时间：
2000-07-24
期刊：
影响因子：
--
通讯作者：
Gunawardena S
中科院分区：
文献类型：
--
作者：
Goldstein LS;Gunawardena S
果蝇发达的遗传学使其成为了解细胞骨架功能和组织机制以及细胞骨架如何与发育和分化相结合的优秀系统。多年的研究表明，果蝇具有一组与大多数其他真核生物性质相似的细胞骨架结构蛋白和蛋白马达。然而，由于缺乏完整的基因组序列，细胞骨架元件的完整工具箱以及它们与其他生物体中细胞骨架蛋白的相似程度仍然未知。随着果蝇常染色质基因组完整序列的最新发布（Adams 等，2000；Rubin 等，2000），我们可以开始编制细胞骨架成分的完整列表。我们还可以一劳永逸地回答以下问题：在哺乳动物和其他真核生物中发现的哪些细胞骨架蛋白存在于果蝇中，以及这些已知蛋白存在哪些变异。本文对果蝇的细胞骨架基因进行了简要回顾，并重点介绍了它们的性质和组织的一些以前未知的有趣特征。正如所描述的（Adams 等人，2000），使用了几种不同的方法来预测果蝇基因组中编码的蛋白质组。这些方法依赖于将序列与已知遗传位点、EST 序列和基因预测程序耦合的组合。使用来自脊椎动物、线虫和酿酒酵母的超过 1,000 个细胞骨架查询序列（参见 http://www.jcb.org/cgi/content/full/150/2/F63/DC1 上的表 SI）搜索预测的蛋白质。通过从 Kreis 和 Vale (1999) 中描述的每一类已知细胞骨架蛋白中选择代表性序列，以及选择秀丽隐杆线虫和酿酒酵母蛋白质组网站上分类为细胞骨架的蛋白质来选择查询。此外，还使用了来自 BLOCKS 数据库的共有序列元素（Henikoff 等人，1999a，b）。搜索主要使用 BLASTP 完成
The well-developed genetics of Drosophila make it an excellent system to understand mechanisms of cytoskeletal function and organization and how the cytoskeleton is coupled to development and differentiation. Many years of work have revealed that Drosophila has a set of cytoskeletal structural proteins and protein motors qualitatively similar to most other eukaryotes. The full toolbox of cytoskeletal elements, and the extent to which they resemble cytoskeletal proteins in other organisms has, however, remained unknown owing to the lack of a complete genome sequence. With the recent arrival of the complete sequence of the euchromatic genome of Drosophila (Adams et al., 2000; Rubin et al., 2000), we can begin to compile a complete list of cytoskeletal components. We can also answer once and for all the question of what cytoskeletal proteins discovered in mammals and other eukaryotes are present in the fly, and what variations on these known proteins exist. This article provides a brief review of the cytoskeletal genes of Drosophila, and highlights several interesting features of their nature and organization not previously known.As described (Adams et al., 2000) several different methods were used to predict the set of proteins encoded in the Drosophila genome. These methods relied on a combination of coupling the sequence to known genetic loci, EST sequences, and gene prediction programs. The predicted proteins were searched using greater than 1,000 cytoskeletal query sequences (see Table SI at http://www. jcb. org/cgi/content/full/150/2/F63/DC1) from vertebrates, C. elegans, and S. cerevisiae. Queries were chosen by selecting representative sequences from each class of known cytoskeletal proteins described in Kreis and Vale (1999), as well as selections of proteins classified as cytoskeletal on the C. elegans and S. cerevisiae proteome Web sites. In addition, consensus sequence elements from the BLOCKS database were also used (Henikoff et al., 1999a, b). Searches were done primarily using BLASTP
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影响因子：
14.9
作者：
Henikoff, JG;Henikoff, S;Pietrokovski, S
通讯作者：
Pietrokovski, S
影响因子：
64.5
作者：
Yang, YM;Bauer, C;Fuchs, E
通讯作者：
Fuchs, E
影响因子：
7.8
作者：
Leung, C L;Sun, D;Zheng, M;Knowles, D R;Liem, R K
通讯作者：
Liem, R K
DOI：
10.1083/jcb.111.6.2563
发表时间：
1990-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Irminger-Finger I;Laymon RA;Goldstein LS
通讯作者：
Goldstein LS
DOI：
10.1073/pnas.95.25.14745
发表时间：
1998-12-08
影响因子：
11.1
作者：
Zheng, PZ;Eastman, J;Pimplikar, SW
通讯作者：
Pimplikar, SW