The gingipains from Porphyromonas gingivalis do not directly induce osteoclast differentiation in primary mouse bone marrow cultures.
The gingipains from Porphyromonas gingivalis do not directly induce osteoclast differentiation in primary mouse bone marrow cultures.
复制标题
来自牙龈卟啉单胞菌的牙龈蛋白酶不会直接诱导原代小鼠骨髓培养物中的破骨细胞分化。
DOI：
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复制
发表时间：
2009
影响因子：
3.5
通讯作者：
R. Pike
中科院分区：
文献类型：
--
作者：
R. Fitzpatrick;P. Campbell;S. Sivagurunathan;C. Pagel;J. Potempa;E. Mackie;R. Pike
背景与目的
BACKGROUND AND OBJECTIVE
Porphyromonas gingivalis is a major aetiological agent in the development of periodontitis, the major clinical hallmark of which is bone resorption. The cysteine proteases (gingipains) produced by P. gingivalis have a critical role in the pathogenesis of the disease, and previous studies on whole bacteria have implicated these enzymes in osteoclastogenesis, a process which serves to upregulate bone resorption. The effects of the gingipains from P. gingivalis on osteoclast differentiation were investigated here to determine whether the enzymes directly contribute to osteoclastogenesis and thus to bone resorption.
MATERIAL AND METHODS
The effects of the gingipains on osteoclast differentiation were investigated in primary mouse bone marrow cultures. The cultures harvested from C57BL6/J mice were incubated in the presence of parathyroid hormone, a known osteoclastogenic factor, or active/inactivated forms of three gingipains. Osteoclast differentiation was quantified by counting the number of multinucleated cells positive for tartrate-resistant acid phosphatase, an enzyme marker for these cells.
RESULTS
After 10 days of culture, the gingipains, either active or inactive, failed to stimulate osteoclast differentiation in comparison to the parathyroid hormone.
CONCLUSION
The data presented here demonstrate that the gingipains do not induce osteoclast differentiation in this system, indicating that the bacterium uses other mechanisms to induce bone loss.
影响因子：
18.6
作者：
Haffajee, A D;Socransky, S S
通讯作者：
Socransky, S S