Overexpression of the Maize ZmNLP6 and ZmNLP8 Can Complement the Arabidopsis Nitrate Regulatory Mutant nlp7 by Restoring Nitrate Signaling and Assimilation.
Overexpression of the Maize ZmNLP6 and ZmNLP8 Can Complement the Arabidopsis Nitrate Regulatory Mutant nlp7 by Restoring Nitrate Signaling and Assimilation.
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DOI：
10.3389/fpls.2017.01703
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发表时间：
2017
影响因子：
5.6
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Cao H;Qi S;Sun M;Li Z;Yang Y;Crawford NM;Wang Y
硝酸盐是影响玉米生长和产量的一种关键营养物质，对玉米硝酸盐调控基因和机制的研究还很少。在此，我们鉴定了玉米中的9个ZmNLP基因，并分析了其中两个成员在硝酸盐信号转导中的功能。定量聚合酶链式反应结果显示，ZmNLP基因在不同时期和不同器官中有广泛的表达模式，其中ZmNLP6和ZmNLP8的转录表达水平最高。当ZmNLP6和ZmNLP8在拟南芥硝酸盐调控基因突变体nlp7-4中过表达时，转基因植株的硝酸盐同化和硝酸盐反应基因的诱导恢复到WT水平，表明ZmNLP6和ZmNLP8可以取代主要的硝酸盐调控基因AtNLP7在硝酸盐信号和代谢中的重要作用。ZmNLP6和ZmNLP8定位于细胞核，在体外可以与候选的硝酸盐应答顺式元件结合。在低硝酸盐条件下，高表达ZmNLP6和ZmNLP8的转基因拟南芥的生物量和产量显著高于野生型和nlp7-4突变体。因此，ZmNLP6和ZmNLP8调控转基因拟南芥植株中的硝酸盐信号转导，可能是提高玉米氮素利用效率的潜在候选者。
Nitrate is a key nutrient that affects maize growth and yield, and much has yet to be learned about nitrate regulatory genes and mechanisms in maize. Here, we identified nine ZmNLP genes in maize and analyzed the functions of two ZmNLP members in nitrate signaling. qPCR results revealed a broad pattern of expression for ZmNLP genes in different stages and organs with the highest levels of transcript expression of ZmNLP6 and ZmNLP8. When ZmNLP6 and ZmNLP8 were overexpressed in the Arabidopsis nitrate regulatory gene mutant nlp7-4, nitrate assimilation and induction of nitrate-responsive genes in the transgenic plants were recovered to WT levels, indicating that ZmNLP6 and ZmNLP8 can replace the essential roles of the master nitrate regulatory gene AtNLP7 in nitrate signaling and metabolism. ZmNLP6 and ZmNLP8 are localized in the nucleus and can bind candidate nitrate-responsive cis-elements in vitro. The biomass and yield of transgenic Arabidopsis lines overexpressing ZmNLP6 and ZmNLP8 showed significant increase compared with WT and nlp7-4 mutant line in low nitrate conditions. Thus, ZmNLP6 and ZmNLP8 regulate nitrate signaling in transgenic Arabidopsis plants and may be potential candidates for improving nitrogen use efficiency of maize.
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影响因子：
5.6
作者：
Garnett T;Plett D;Conn V;Conn S;Rabie H;Rafalski JA;Dhugga K;Tester MA;Kaiser BN
通讯作者：
Kaiser BN
影响因子：
64.8
作者：
Liu KH;Niu Y;Konishi M;Wu Y;Du H;Sun Chung H;Li L;Boudsocq M;McCormack M;Maekawa S;Ishida T;Zhang C;Shokat K;Yanagisawa S;Sheen J
通讯作者：
Sheen J
影响因子：
9.4
作者：
Garnett, Trevor;Conn, Vanessa;Kaiser, Brent N.
通讯作者：
Kaiser, Brent N.
影响因子：
16.6
作者：
Marchive, Chloe;Roudier, Francois;Krapp, Anne
通讯作者：
Krapp, Anne
影响因子：
7.4
作者：
Ferrario-Méry, S;Valadier, MH;Foyer, CH
通讯作者：
Foyer, CH