Age-associated DNA methylation changes in Xenopus frogs.
Age-associated DNA methylation changes in Xenopus frogs.
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DOI：
10.1080/15592294.2023.2201517
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发表时间：
2023-12
期刊：
影响因子：
3.7
通讯作者：
Pellegrini, Matteo
中科院分区：
文献类型：
--
作者：
Morselli, Marco;Bennett, Ronan;Shaidani, Nikko-Ideen;Horb, Marko;Peshkin, Leonid;Pellegrini, Matteo
关键词：
DNA甲基化与年龄相关的变化已经在各种动物中得到了表征，但在两栖动物中还没有，两栖动物特别令人感兴趣，因为它们包括被广泛研究的模式生物。在这项研究中，我们提出了明确的证据，证明热带水生脊椎动物非洲爪哇在DNA甲基化方面表现出与年龄相关的变化模式。我们从代表年轻、成熟和老年的九种青蛙的皮肤样本中生成了全基因组亚硫酸盐测序(WGBS)图谱，并表征了基因和染色体规模的DNA甲基化随年龄的变化。我们观察到的许多甲基化特征和变化与哺乳动物中已知的一致，表明与年龄相关的变化机制是保守的。此外，我们选择了几千个与年龄相关的CpG位点来建立基于靶向DNA甲基化分析(TBSseq)的分析，以在未来涉及更大个体队列的研究中扩大我们的发现。TBSeq试点实验的初步结果概括了用WGBS获得的发现，为表观遗传学时钟分析的发展奠定了基础。这项研究的结果将使我们能够利用非洲爪哇可用的独特资源来研究DNA甲基化如何与其他衰老特征相关。
Age-associated changes in DNA methylation have been characterized across various animals, but not yet in amphibians, which are of particular interest because they include widely studied model organisms. In this study, we present clear evidence that the aquatic vertebrate species Xenopus tropicalis displays patterns of age-associated changes in DNA methylation. We have generated whole-genome bisulfite sequencing (WGBS) profiles from skin samples of nine frogs representing young, mature, and old adults and characterized the gene- and chromosome-scale DNA methylation changes with age. Many of the methylation features and changes we observe are consistent with what is known in mammalian species, suggesting that the mechanism of age-related changes is conserved. Moreover, we selected a few thousand age-associated CpG sites to build an assay based on targeted DNA methylation analysis (TBSseq) to expand our findings in future studies involving larger cohorts of individuals. Preliminary results of a pilot TBSeq experiment recapitulate the findings obtained with WGBS setting the basis for the development of an epigenetic clock assay. The results of this study will allow us to leverage the unique resources available for Xenopus to study how DNA methylation relates to other hallmarks of ageing.
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影响因子：
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期刊：
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