SEARCHGTr: a program for analysis of glycosyltransferases involved in glycosylation of secondary metabolites.
SEARCHGTr: a program for analysis of glycosyltransferases involved in glycosylation of secondary metabolites.
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DOI：
10.1093/nar/gki449
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发表时间：
2005-07-01
影响因子：
14.9
通讯作者：
Mohanty D
中科院分区：
文献类型：
--
作者：
Kamra P;Gokhale RS;Mohanty D
SEARCHGTr 是一款基于网络的软件，用于分析糖基转移酶 (GTrs)，该酶参与多种药学上重要的化合物（如阿霉素、红霉素、万古霉素等）的生物合成。该软件是基于对来自 52 个天然产物生物合成基因簇的 102 个已知特异性的 GTr 的序列/结构特征的综合分析而开发的。 SEARCHGTr 是一种强大的工具，可将 GTr 序列与其相应底物的化学结构相关联。该软件指示供体/受体特异性，并识别推定的底物结合残基。此外，它还提供与其他公共数据库（如 GENBANK、SWISS-PROT、CAZY、PDB、PDBSum 和 PUBMED）的接口，用于提取与查询序列同源的 GTrs 的各种信息。 SEARCHGTr 将为我们之前开发的生物信息学工具 NRPS-PKS 提供新的维度。这些工具共同促进了对糖苷配基核心及其下游糖基化生物合成所涉及的蛋白质的全面计算分析。除了为合理设计新型天然产物提供机会外，这些工具还将有助于鉴定新测序的基因组中发现的次生代谢物基因簇的生物合成产物。可以通过以下网址访问 SEARHGTr。
SEARCHGTr is a web-based software for the analysis of glycosyltransferases (GTrs) involved in the biosynthesis of a variety of pharmaceutically important compounds like adriamycin, erythromycin, vancomycin etc. This software has been developed based on a comprehensive analysis of sequence/structural features of 102 GTrs of known specificity from 52 natural product biosynthetic gene clusters. SEARCHGTr is a powerful tool that correlates sequences of GTrs to the chemical structures of their corresponding substrates. This software indicates the donor/acceptor specificity and also identifies putative substrate binding residues. In addition, it provides interfaces to other public databases like GENBANK, SWISS-PROT, CAZY, PDB, PDBSum and PUBMED for extracting various information on GTrs homologous to the query sequence. SEARCHGTr would provide new dimension to our previously developed bioinformatics tool NRPS-PKS. Together, these tools facilitate comprehensive computational analysis of proteins involved in biosynthesis of aglycone core and its downstream glycosylations. Apart from presenting opportunities for rational design of novel natural products, these tools would assist in the identification of biosynthetic products of secondary metabolite gene clusters found in newly sequenced genomes. SEARCHGTr can be accessed at .
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影响因子：
14.9
作者：
Ansari, MZ;Yadav, G;Mohanty, D
通讯作者：
Mohanty, D
影响因子：
14.9
作者：
Laskowski, RA
通讯作者：
Laskowski, RA
影响因子：
--
作者：
Losey, HC;Jiang, JQ;Walsh, CT
通讯作者：
Walsh, CT
影响因子：
--
作者：
Hoffmeister, D;Wilkinson, B;Bechthold, A
通讯作者：
Bechthold, A
影响因子：
16
作者：
Trivedi, OA;Arora, P;Gokhale, RS
通讯作者：
Gokhale, RS