The characterization of high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model.
The characterization of high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model.
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DOI：
10.1155/2008/704045
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发表时间：
2008
影响因子：
--
通讯作者：
Chen, Li
中科院分区：
文献类型：
--
作者：
Zhang, Ming;Lv, Xiao-Yan;Li, Jing;Xu, Zhi-Gang;Chen, Li
的目标。在之前建立的方法的基础上，我们通过高脂肪饮食联合多次低剂量STZ注射建立了一个更好、更稳定的2型糖尿病动物模型。同时，利用该模型评价小檗碱的抗糖尿病作用。方法。常规饲料喂养4周的Wistar雄性大鼠给药（对照组），高脂饲料喂养4周的大鼠腹腔注射1次或2次不同剂量的STZ（糖尿病模型组），糖尿病大鼠给药小檗碱（100 mg/kg，小檗碱治疗组）。进行腹腔葡萄糖耐量试验和胰岛素耐量试验。同时测定空腹血糖、空腹胰岛素、总胆固醇和甘油三酯，评价动态血糖和脂质代谢。结果。单次注射45 mg/kg STZ组大鼠成功率最高（100%），但血浆胰岛素水平明显降低，ISI与对照组无显著差异。30 mg/kg STZ 2次注射组成功率显著高（85%），表现为胰岛素抵抗、高血糖、血脂紊乱等典型的T2DM特征。30 mg/kg STZ 2次注射组经小檗碱治疗后症状均恢复。结论。综上所述，高脂肪饮食联合多次低剂量STZ （30 mg/kg，每周间隔2周）是建立稳定的2型糖尿病动物模型的较好方法，该模型可能适合药物筛选。
Aim. Based on the previously established method, we developed a better and stable animal model of type 2 diabetes mellitus by high-fat diet combined with multiple low-dose STZ injections. Meanwhile, this new model was used to evaluate the antidiabetic effect of berberine. Method. Wistar male rats fed with regular chow for 4 weeks received vehicle (control groups), rats fed with high-fat diet for 4 weeks received different amounts of STZ once or twice by intraperitoneal injection (diabetic model groups), and diabetic rats were treated with berberine (100 mg/kg, berberine treatment group). Intraperitoneal glucose tolerance test and insulin tolerance test were carried out. Moreover, fasting blood glucose, fasting insulin, total cholesterol, and triglyceride were measured to evaluate the dynamic blood sugar and lipid metabolism. Result. The highest successful rate (100%) was observed in rats treated with a single injection of 45 mg/kg STZ, but the plasma insulin level of this particular group was significantly decreased, and ISI has no difference compared to control group. The successful rate of 30 mg/kg STZ twice injection group was significantly high (85%) and the rats in this group presented a typical characteristic of T2DM as insulin resistance, hyperglycemia, and blood lipid disorder. All these symptoms observed in the 30 mg/kg STZ twice injection group were recovered by the treatment of berberine. Conclusion. Together, these results indicated that high-fat diet combined with multiple low doses of STZ (30 mg/kg at weekly intervals for 2 weeks) proved to be a better way for developing a stable animal model of type 2 diabetes, and this new model may be suitable for pharmaceutical screening.
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影响因子：
9.8
作者：
Yin, J;Hu, RM;Chen, JL
通讯作者：
Chen, JL
影响因子：
7.7
作者：
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DOI：
10.1111/j.1439-0396.2007.00723.x
发表时间：
2008-02-01
影响因子：
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作者：
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通讯作者：
Gleichmann, H