Heat shock response of Saccharomyces cerevisiae mutants altered in cyclic AMP-dependent protein phosphorylation
Heat shock response of Saccharomyces cerevisiae mutants altered in cyclic AMP-dependent protein phosphorylation
复制标题
酿酒酵母突变体的热激反应在环 AMP 依赖性蛋白磷酸化中发生改变
DOI：
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发表时间：
1987
影响因子：
5.3
通讯作者：
T. Ishikawa
中科院分区：
文献类型：
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作者：
Deug Y. Shin;'. K. Matsumoto;H. Iida;T. Uno;T. Ishikawa
当在23 ℃下生长的酿酒酵母细胞被转移到36 ℃时，它们开始合成热休克蛋白，获得对温度变化后给予的致死热处理的耐热性，并在细胞分裂周期的G1期短暂地阻止它们的生长。bcy 1突变体，导致生产环腺苷酸（cAMP）-独立的蛋白激酶不合成三个热休克蛋白hsp 72 A，hsp 72 B，和hsp 41后的温度变化。bcy 1细胞未能获得对致死热处理的热耐受性，并且在温度变化后未被停滞在G1期。与此相反，产生低水平cAMP的突变体，其组成型产生三种热休克蛋白和四种其他蛋白质，而没有温度变化，并且对致死热处理具有抗性。结果表明，cAMP依赖性蛋白磷酸化水平的降低导致热休克反应，包括三种热休克蛋白的合成升高，热耐受性的获得和细胞周期的短暂停滞。
When Saccharomyces cerevisiae cells grown at 23 degrees C were transferred to 36 degrees C, they initiated synthesis of heat shock proteins, acquired thermotolerance to a lethal heat treatment given after the temperature shift, and arrested their growth transiently at the G1 phase of the cell division cycle. The bcy1 mutant which resulted in production of cyclic AMP (cAMP)-independent protein kinase did not synthesize the three heat shock proteins hsp72A, hsp72B, and hsp41 after the temperature shift. The bcy1 cells failed to acquire thermotolerance to the lethal heat treatment and were not arrested at the G1 phase after the temperature shift. In contrast, the cyr1-2 mutant, which produced a low level of cAMP, constitutively produced three heat shock proteins and four other proteins without the temperature shift and was resistant to the lethal heat treatment. The results suggest that a decrease in the level of cAMP-dependent protein phosphorylation results in the heat shock response, including elevated synthesis of three heat shock proteins, acquisition of thermotolerance, and transient arrest of the cell cycle.
DOI：
10.1073/pnas.79.10.3218
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
LI, GC;WERB, Z
通讯作者：
WERB, Z