Human Skin Constructs with Spatially Controlled Vasculature Using Primary and iPSC-Derived Endothelial Cells.
Human Skin Constructs with Spatially Controlled Vasculature Using Primary and iPSC-Derived Endothelial Cells.
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DOI：
10.1002/adhm.201500936
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发表时间：
2016-07
影响因子：
10
通讯作者：
Christiano, Angela M.
中科院分区：
文献类型：
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作者：
Abaci, Hasan E.;Guo, Zongyou;Coffman, Abigail;Gillette, Brian;Lee, Wen-han;Sia, Samuel K.;Christiano, Angela M.
工程化人类皮肤构建物的血管化对于再现全身性药物递送以及对于它们的长期存活、功能性和可行的植入是至关重要的。在这项研究中，我们使用最新的微细加工技术，并建立了一种新型生物工程方法，使用原代和诱导多能干细胞（iPSC）衍生的内皮细胞（EC）在3D人体皮肤等效物中微图案化空间控制和可灌注的血管网络。使用3D打印技术，我们能够控制微图案化血管网络的几何形状。我们证实，血管化的人皮肤等同物（vHSE）可以形成一个强大的表皮，并建立内皮屏障功能，这使得局部和全身药物输送的重演。此外，我们研究了vHSEs对免疫缺陷小鼠皮肤伤口的治疗潜力，并证明vHSEs可以促进和引导伤口愈合过程中的新血管形成。总的来说，这种创新的生物工程方法可以实现局部和全身药物递送的体外评估，以及提高工程皮肤构建体用作治疗皮肤伤口的潜在治疗选择的潜力。
Vascularization of engineered human skin constructs is crucial for recapitulation of systemic drug delivery and for their long-term survival, functionality, and viable engraftment. In this study, we used the latest microfabrication techniques and established a novel bioengineering approach to micropattern spatially-controlled and perfusable vascular networks in 3D human skin equivalents using both primary and induced pluripotent stem cell (iPSC)-derived endothelial cells (ECs). Using 3D printing technology, we were able to control the geometry of the micropatterned vascular networks. We verified that vascularized human skin equivalents (vHSEs) can form a robust epidermis and establish an endothelial barrier function, which allows for the recapitulation of both topical and systemic delivery of drugs. In addition, we examined the therapeutic potential of vHSEs for cutaneous wounds on immunodeficient mice and demonstrated that vHSEs can both promote and guide neovascularization during wound healing. Overall, this innovative bioengineering approach could enable in vitro evaluation of topical and systemic drug delivery, as well as improve the potential of engineered skin constructs to be used as a potential therapeutic option for the treatment of cutaneous wounds.
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影响因子：
4.6
作者：
Egawa, Gyohei;Nakamizo, Satoshi;Natsuaki, Yohei;Doi, Hiromi;Miyachi, Yoshiki;Kabashima, Kenji
通讯作者：
Kabashima, Kenji
影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
影响因子：
3
作者：
Maione, Anna G.;Brudno, Yevgeny;Garlick, Jonathan A.
通讯作者：
Garlick, Jonathan A.
影响因子：
9
作者：
Chau, David Y. S.;Johnson, Claire;Ghaemmaghami, Amir M.
通讯作者：
Ghaemmaghami, Amir M.
影响因子：
41.2
作者：
通讯作者：
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