Identification of genes regulating ovary differentiation after pollination in hazel by comparative transcriptome analysis.
Identification of genes regulating ovary differentiation after pollination in hazel by comparative transcriptome analysis.
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通过比较转录组分析鉴定榛子授粉后调节子房分化的基因
DOI：
10.1186/s12870-018-1296-3
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发表时间：
2018-05-09
影响因子：
5.3
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Cheng Y;Zhang Y;Liu C;Ai P;Liu J
背景榛子(Corylusspp.)表现出授粉后从子房原基开始的子房分化和发育，使植物具有独特的延迟受精。授粉后子房和胚珠发育不良可分别导致子房败育和空果形成，从而造成产量损失。然而，参与子房和胚珠分化发育的基因在很大程度上是未知的。结果在未授粉的雌花序(F期)中，柱头呈伸展生长模式。授粉后，发育的子房开始形成(S期)，随后是胚珠分化(T期)和生长(FO期)。从F期、S期、T期和FO期的雌花或幼子房中提取总RNA，在HiSeq4000系统上进行测序。测序结果显示，F-vs-S、S-VS-T和T-vs-FO的差异表达转录本分别为5524、3468和8714个。根据京都基因和基因组百科全书的注释，对F-VS-S、S-VS-T和T-VS-FO的配对比较分析发现，有6条途径在卵巢分化过程中显著丰富，包括Ko04075(植物激素信号转导)。授粉后生长素水平升高，免疫组织化学分析表明，生长素在雌花序和幼子房的生长中心富含生长素。这些结果表明，与生长素生物合成、运输、信号转导、花四元组模型和花发育相关的基因可能调控着榛子的子房和胚珠的分化和发育。结论本研究结果为揭示该属植物授粉后子房分化和发育的分子机制提供了依据。
BackgroundHazel (Corylusspp.) exhibits ovary differentiation and development that is initiated from the ovary primordium after pollination, conferring the plant with a unique delayed fertilization. Failure of development of the ovary and ovule after pollination can lead to ovary abortion and blank fruit formation, respectively, with consequent yield loss. However, the genes involved in ovary and ovule differentiation and development are largely unknown.ResultsIn unpollinated pistillate inflorescences (stage F), the stigma shows an extension growth pattern. After pollination, a rudimentary ovary begins to form (stage S), followed by ovule differentiation (stage T) and growth (stage FO). Total RNA was obtained from pistillate inflorescences or young ovaries at stage F, S, T and FO, and sequencing was carried out on a HiSeq 4000 system. De novo assembly of sequencing data yielded 62.58 Gb of nucleotides and 90,726 unigenes; 5524, 3468, and 8714 differentially expressed transcripts were identified in F-vs-S, S-vs-T, and T-vs-FO paired comparisons, respectively. An analysis of F-vs-S, S-vs-T, and T-vs-FO paired comparisons based on annotations in the Kyoto Encyclopedia of Genes and Genomes revealed six pathways that were significantly enriched during ovary differentiation, including ko04075 (Plant hormone signal transduction). Auxin level increased after pollination, and an immunohistochemical analysis indicated that auxin was enriched at the growth center of pistillate inflorescences and young ovaries. These results indicate that genes related to auxin biosynthesis, transport, signaling, the floral quartet model, and flower development may regulate ovary and ovule differentiation and development in hazel.ConclusionsOur findings provide insight into the molecular mechanisms of ovary differentiation and development after pollination in this economically valuable plant.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
4
作者：
Blazquez, MA
通讯作者：
Blazquez, MA
影响因子：
4.2
作者：
Ciampolini, F;Cresti, M
通讯作者：
Cresti, M
发育和败育榛子胚珠植物激素生物合成和信号转导途径的比较
影响因子：
4.2
作者：
Cheng, Yunqing;Zhao, Yixin;Ming, Yuetong
通讯作者：
Ming, Yuetong
影响因子：
11.4
作者：
Mallory, AC;Reinhart, BJ;Bartel, DP
通讯作者：
Bartel, DP