Multiple P450s and Variation in Neuronal Genes Underpins the Response to the Insecticide Imidacloprid in a Population of Drosophila melanogaster.
Multiple P450s and Variation in Neuronal Genes Underpins the Response to the Insecticide Imidacloprid in a Population of Drosophila melanogaster.
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DOI：
10.1038/s41598-017-11092-5
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发表时间：
2017-09-12
影响因子：
4.6
通讯作者：
Batterham P
中科院分区：
文献类型：
--
作者：
Denecke S;Fusetto R;Martelli F;Giang A;Battlay P;Fournier-Level A;O' Hair RA;Batterham P
杀虫剂抗性是对强烈的选择压力作出反应的进化的一个经济上重要的例子。本文利用果蝇遗传参考小组（Drosophila Genetic Reference Panel）对新烟碱类杀虫剂吡虫啉（imidacloprid）的抗性遗传学进行了研究，该小组收集了来自北卡罗来纳州单一种群的近交黑腹果蝇基因型。不同基因型对吡虫啉的抗性差异很大，抗性越强的基因型对吡虫啉的代谢和排泄能力越强。抗性水平的变化与基因组和转录组学的变化有关，暗示了几个候选基因参与中枢神经系统功能和细胞色素p450 Cyp6g1和Cyp6g2。CRISPR-Cas9介导的Cyp6g1的去除表明，它仅在已经高度表达的背景下促成吡虫啉耐药性。Cyp6g2，先前通过环腺的表达参与幼代激素的合成，被证明在抗性基因型的代谢相关组织中表达。Cyp6g2过表达既能代谢吡虫啉，又能产生耐药性。这些数据共同表明，吡虫啉耐药性受到多种已知和未知遗传因素的影响。
Insecticide resistance is an economically important example of evolution in response to intense selection pressure. Here, the genetics of resistance to the neonicotinoid insecticide imidacloprid is explored using the Drosophila Genetic Reference Panel, a collection of inbred Drosophila melanogaster genotypes derived from a single population in North Carolina. Imidacloprid resistance varied substantially among genotypes, and more resistant genotypes tended to show increased capacity to metabolize and excrete imidacloprid. Variation in resistance level was then associated with genomic and transcriptomic variation, implicating several candidate genes involved in central nervous system function and the cytochrome P450s Cyp6g1 and Cyp6g2. CRISPR-Cas9 mediated removal of Cyp6g1 suggested that it contributed to imidacloprid resistance only in backgrounds where it was already highly expressed. Cyp6g2, previously implicated in juvenile hormone synthesis via expression in the ring gland, was shown to be expressed in metabolically relevant tissues of resistant genotypes. Cyp6g2 overexpression was shown to both metabolize imidacloprid and confer resistance. These data collectively suggest that imidacloprid resistance is influenced by a variety of previously known and unknown genetic factors.
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影响因子：
3.7
作者：
Denecke S;Nowell CJ;Fournier-Level A;Perry T;Batterham P
通讯作者：
Batterham P
DOI：
10.1534/g3.116.031054
发表时间：
2016-08-09
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Battlay P;Schmidt JM;Fournier-Level A;Robin C
通讯作者：
Robin C
影响因子：
30.8
作者：
Ayroles, Julien F.;Carbone, Mary Anna;Stone, Eric A.;Jordan, Katherine W.;Lyman, Richard F.;Magwire, Michael M.;Rollmann, Stephanie M.;Duncan, Laura H.;Lawrence, Faye;Anholt, Robert R. H.;Mackay, Trudy F. C.
通讯作者：
Mackay, Trudy F. C.
影响因子：
9.8
作者：
Foley E;O'Farrell PH
通讯作者：
O'Farrell PH
DOI：
10.1073/pnas.1519159112
发表时间：
2015-11-03
影响因子：
11.1
作者：
Huang, Wen;Carbone, Mary Anna;Mackay, Trudy F. C.
通讯作者：
Mackay, Trudy F. C.