Creatine kinase isoforms following isometric exercise
Creatine kinase isoforms following isometric exercise
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等长运动后的肌酸激酶亚型
DOI：
10.1002/mus.880100108
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发表时间：
1987
期刊：
影响因子：
3.4
通讯作者：
P. Triffletti
中科院分区：
文献类型：
--
作者：
P. Clarkson;F. Apple;W. Byrnes;K. McCormick;P. Triffletti
本研究评估了肌酸激酶（CK）活性，CK MM亚型，和肌肉酸痛后的运动方案，旨在诱导骨骼肌损伤。8名大学年龄受试者进行了40次膝伸肌最大等长收缩（10秒收缩/20秒休息）。在运动前和运动后2、6、18和24小时采集血清样品和疼痛评级。通过平板等电聚焦（IEF）测定CK MM 1和CK MM 3亚型。在血清中，MM 1亚型（纯基因产物）被认为是肌肉新释放CK的证据，因为在进入血浆后，CK MM 1转化为MM 2，然后转化为MM 3。在6小时发现血清CK活性显著增加，并在24小时保持升高。CK MM 1在2小时显著升高，在6小时达到峰值，然后接近基线。疼痛直到18小时才显著增加。血清中CK亚型的分析可以提供比总CK或疼痛感更早的骨骼肌损伤指标，并且可以用于跟踪骨骼肌损伤和修复的时间过程。
The present study assessed creatine kinase (CK) activity, CK MM isoforms, and muscle soreness following an exercise regimen designed to induce skeletal muscle damage. Eight college‐age subjects performed 40 maximal isometric contractions of the knee extensor muscles (10‐second contraction/20‐second rest). Serum samples and soreness ratings were taken prior to and 2, 6, 18, and 24 hours after the exercise. The CK MM1 and CK MM3 isoforms were determined by flatbed isoelectric focusing (IEF). In serum, the MM1 isoform (the pure gene product) is considered to be evidence of newly released CK from muscle, as upon entering the plasma, the CK MM1 is converted to MM2 and then MM3. A significant increase in serum CK activity was found at 6 hours and remained elevated at 24 hours. CK MM1 increased significantly at 2 hours, peaked at 6 hours, then approached baseline. Soreness did not increase significantly until 18 hours. Analysis of CK isoforms in serum can provide an earlier indicator of skeletal muscle damage than total CK or perception of soreness and may be useful in tracking the time course of skeletal muscle damage and repair.
通过分析血浆 MM 肌酸激酶亚型早期检测清醒犬的心肌梗塞。
影响因子：
37.8
作者：
Hashimoto,H;Abendschein,DR;Strauss,AW;Sobel,BE
通讯作者：
Sobel,BE