Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)
Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)
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响应Sporisorium reilianum f 的基因表达谱分析。
DOI：
10.1016/j.ejbt.2014.07.006
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发表时间：
2014-09
影响因子：
2.7
通讯作者：
Kan SS
中科院分区：
文献类型：
--
作者：
Yu T;Wang ZH;Jin XC;Liu XJ;Kan SS
玉米丝黑穗病是由玉米丝黑穗病菌Sporisorium reilipentdf.玉米大斑病菌（Sp.zeae（Kühn））是玉米上的一种严重病害。为了揭示玉米抗丝黑穗病的分子机制，利用含有14，850个探针的基因芯片，对玉米抗丝黑穗病近等基因系（NIL）和高感自交系S.结果3,532个基因的表达水平在接种后发生了变化，占探针总数的23.8%。基因本体分析表明，差异表达基因参与了生理生化途径。京都基因与基因组百科全书（KEGG）途径分析表明，植物与病原菌互作、自然杀伤细胞介导的细胞毒作用和苯并恶嗪类化合物生物合成途径在抗丝黑穗病中起重要作用。3个丝黑穗病抗性相关的候选基因，CLAVATA 1，类固醇不敏感1相关受体激酶1和LOC 100217307与亮氨酸丰富的重复序列（LRR）保守结构域被确定，其中每一个都在玉米定位bin 2.09，一个区域先前被证明包括一个主要的QTL的丝黑穗病抗性。此外，通过实时荧光定量PCR（qRT）验证了LOC 100217307基因的正确性，推测该基因可能与玉米丝黑穗病的抗性有关。
BackgroundHead smut of maize, which is caused bySporisorium reilianumf. sp.zeae(Kühn), is a serious disease in maize. In order to reveal the molecular mechanism of the resistance to head smut in maize, a microarray containing ∼ 14,850 probes was used to monitor the gene expression profiles between a disease resistant near isogenic line (NIL) and a highly susceptible inbred line afterS. reilianumwas injected with an artificial inoculation method.ResultsLevels of expression for 3,532 genes accounting for 23.8% of the total probes changed after inoculation. Gene Ontology analysis revealed that the differentially expressed genes participated in physiological and biochemical pathways. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that plant–pathogen interaction, natural killer cell mediated cytotoxicity and benzoxazinoid biosynthesis pathways play important roles in resistance to head smut. Three head smut resistance-related candidate genes, CLAVATA1, bassinosteroid insensitive 1-associated receptor kinase 1 and LOC100217307 with leucine-rich repeat (LRR) conserved domains were identified, each of which is in maize mapping bin 2.09, a region previously shown to include a major QTL for head smut resistance. Furthermore, LOC100217307 was validated by quantitative real-time (qRT)-PCR inferring that this gene may be involved in the resistance to head smut of maize.ConclusionsThis study provided valuable information for cloning, functional analysis and marker assisted breeding of head smut resistance genes.
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影响因子：
3.2
作者：
I. A. Al-Sohailv;C. J. Mankin;G. Semeniuk
通讯作者：
I. A. Al-Sohailv;C. J. Mankin;G. Semeniuk
影响因子：
6.9
作者：
Rodríguez-Serrano M;Bárány I;Prem D;Coronado MJ;Risueño MC;Testillano PS
通讯作者：
Testillano PS
影响因子：
11.6
作者：
Leonhardt, N;Kwak, JM;Schroeder, JI
通讯作者：
Schroeder, JI
DOI：
--
发表时间：
2007
期刊：
Journal of Plant Genetic Resources
影响因子：
--
作者：
Hao Zhuan-fang
通讯作者：
Hao Zhuan-fang
DOI：
--
发表时间：
2000
期刊：
Maize Sciences
影响因子：
--
作者：
Zhang Xiu-wen
通讯作者：
Zhang Xiu-wen