Simultaneous Aerosol and Intramuscular Immunization with Influenza Vaccine Induces Powerful Protective Local T Cell and Systemic Antibody Immune Responses in Pigs.
Simultaneous Aerosol and Intramuscular Immunization with Influenza Vaccine Induces Powerful Protective Local T Cell and Systemic Antibody Immune Responses in Pigs.
复制标题
DOI：
10.4049/jimmunol.2001086
复制
发表时间：
2021-02-01
期刊：
影响因子：
--
通讯作者：
Tchilian E
中科院分区：
文献类型：
--
作者：
Martini V;Paudyal B;Chrun T;McNee A;Edmans M;Atangana Maze E;Clark B;Nunez A;Dolton G;Sewell A;Beverley P;MacLoughlin R;Townsend A;Tchilian E
同时气雾剂和i.m.免疫减少病毒载量和病理学。同时免疫诱导强保护性局部和全身反应。血液中NP特异性T细胞的层次结构并不反映局部肺反应。提供针对流感病毒的强大Ab和交叉反应性T细胞免疫应答的疫苗将对人类和猪都有益。在这项研究中，我们评估了i.m.，气溶胶（Aer），并在Babraham猪中通过两种途径同时进行全身和呼吸道免疫（SIM），使用单周期候选流感疫苗S-FLU。在初免和加强免疫后，用H1N1 pdm 09病毒攻击猪。i. m. -免疫猪产生高滴度的中和抗体，但差的T细胞应答，而Aer诱导强有力的呼吸道T细胞应答，但低滴度的抗体。SIM猪结合了高Ab滴度和强局部T细胞应答。SIM显示出对病毒脱落的最完全抑制和病理学的最大改善。我们的结论是SIM制度对呼吸道病原体的免疫值得进一步研究。
Simultaneous aerosol and i.m. immunization reduces viral load and pathology. Simultaneous immunization induces strong protective local and systemic responses. The hierarchy of NP-specific T cells in blood does not reflect local lung responses. A vaccine providing both powerful Ab and cross-reactive T cell immune responses against influenza viruses would be beneficial for both humans and pigs. In this study, we evaluated i.m., aerosol (Aer), and simultaneous systemic and respiratory immunization (SIM) by both routes in Babraham pigs, using the single cycle candidate influenza vaccine S-FLU. After prime and boost immunization, pigs were challenged with H1N1pdm09 virus. i.m.-immunized pigs generated a high titer of neutralizing Abs but poor T cell responses, whereas Aer induced powerful respiratory tract T cell responses but a low titer of Abs. SIM pigs combined high Ab titers and strong local T cell responses. SIM showed the most complete suppression of virus shedding and the greatest improvement in pathology. We conclude that SIM regimes for immunization against respiratory pathogens warrant further study.
登录
查看更多内容
影响因子：
8
作者：
McMaster SR;Wein AN;Dunbar PR;Hayward SL;Cartwright EK;Denning TL;Kohlmeier JE
通讯作者：
Kohlmeier JE
影响因子：
8
作者：
Jeyanathan, M.;Damjanovic, D.;Xing, Z.
通讯作者：
Xing, Z.
影响因子：
6.4
作者：
Mohn, Kristin G. I.;Zhou, Fan;Cox, Rebecca J.
通讯作者：
Cox, Rebecca J.
影响因子：
4.4
作者：
Holzer, Barbara;Morgan, Sophie B.;Tchilian, Elma
通讯作者：
Tchilian, Elma
影响因子：
5.4
作者：
Lau, Yuk-Fai;Wright, Amber R.;Subbarao, Kanta
通讯作者：
Subbarao, Kanta