Capturing structure and function in an embryonic heart with biophotonic tools.
Capturing structure and function in an embryonic heart with biophotonic tools.
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DOI：
10.3389/fphys.2014.00351
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发表时间：
2014
影响因子：
4
通讯作者：
Watanabe M
中科院分区：
文献类型：
--
作者：
Karunamuni GH;Gu S;Ford MR;Peterson LM;Ma P;Wang YT;Rollins AM;Jenkins MW;Watanabe M
早期发育阶段的心功能紊乱与后期的细胞/分子、结构和功能心脏异常相关，最终导致出生时出现先天性心脏缺陷（CHDs）。虽然我们对心脏发育的细胞和分子步骤的了解正在迅速增长，但我们对胚胎中心血管功能作用的理解仍处于早期阶段。这一领域信息匮乏的一个原因是研究早期心脏功能的工具有限。最近开发和适应的生物光子工具可能克服研究微小脆弱的跳动心脏的一些挑战。在本章中，我们描述并讨论了我们在开发和实施生物光子工具来研究功能在心脏发育中的作用方面的经验，重点是光学相干断层扫描（OCT）。OCT可用于生理条件下管状和环状胚胎心脏的详细结构和功能研究。使用OCT可以在早期和后期快速定量地对同一心脏进行表型分析，当与光学制图（OM）和光学起搏（OP）等其他工具结合使用时，OCT有可能在空间和时间上详细揭示影响机械转导途径的生物物理变化。当这些信息与我们对形态发生和所描述的相关分子途径的理解相结合时，可能为冠心病的病因提供更好的解释。讨论了生物光子工具的创造和应用的未来发展方向。
Disturbed cardiac function at an early stage of development has been shown to correlate with cellular/molecular, structural as well as functional cardiac anomalies at later stages culminating in the congenital heart defects (CHDs) that present at birth. While our knowledge of cellular and molecular steps in cardiac development is growing rapidly, our understanding of the role of cardiovascular function in the embryo is still in an early phase. One reason for the scanty information in this area is that the tools to study early cardiac function are limited. Recently developed and adapted biophotonic tools may overcome some of the challenges of studying the tiny fragile beating heart. In this chapter, we describe and discuss our experience in developing and implementing biophotonic tools to study the role of function in heart development with emphasis on optical coherence tomography (OCT). OCT can be used for detailed structural and functional studies of the tubular and looping embryo heart under physiological conditions. The same heart can be rapidly and quantitatively phenotyped at early and again at later stages using OCT. When combined with other tools such as optical mapping (OM) and optical pacing (OP), OCT has the potential to reveal in spatial and temporal detail the biophysical changes that can impact mechanotransduction pathways. This information may provide better explanations for the etiology of the CHDs when interwoven with our understanding of morphogenesis and the molecular pathways that have been described to be involved. Future directions for advances in the creation and use of biophotonic tools are discussed.
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影响因子：
2.5
作者：
Chen, Fuhua;De Diego, Carlos;Chang, Marvin G.;McHarg, Jennifer L.;John, Scott;Klitzner, Thomas S.;Weiss, James N.
通讯作者：
Weiss, James N.
影响因子：
3.3
作者：
Berrios-Otero, Cesar A.;Nieman, Brian J.;Turnbull, Daniel H.
通讯作者：
Turnbull, Daniel H.
影响因子：
20.1
作者：
Briggs, Laura E.;Phelps, Aimee L.;Wessels, Andy
通讯作者：
Wessels, Andy
影响因子：
2.3
作者：
Chen, SY;Periasamy, A;Sulik, KK
通讯作者：
Sulik, KK
DOI：
10.1152/ajpheart.00359.2007
发表时间：
2007-10-01
影响因子：
4.8
作者：
Chen, Fuhua;De Diego, Carlos;Weiss, James N.
通讯作者：
Weiss, James N.