Modulation of the contractility of micropatterned myocardial cells with nanoscale forces using atomic force microscopy
Modulation of the contractility of micropatterned myocardial cells with nanoscale forces using atomic force microscopy
复制标题
使用原子力显微镜以纳米级力调节微图案心肌细胞的收缩性
作者：
N. Nagarajan;Varun Vyas;B. Huey;P. Zorlutuna
调节心肌细胞收缩力的能力对于从心脏病治疗到生物机器人等生物工程应用非常重要。在这项研究中，我们使用原子力显微镜检查了单细胞水平纳米级机械刺激下新生大鼠心肌细胞收缩频率的变化。为了测量相同密度细胞的响应，将它们微图案化成固定几何形状的微片。为了检查基质硬度对细胞行为的影响，将它们分别培养在较硬和较软的表面（玻璃和聚二甲基硅氧烷）上。在 5 Hz、300 nN 的周期性循环刺激下，观察到聚（二甲基硅氧烷）基底上的细胞斑块的同步收缩速率在其自发搏动速率方面显着降低，而玻璃基底上的细胞斑块在刺激后保持或增加其收缩速率。另一方面，与微图案细胞贴片相比，单个细胞大多保持其收缩率，并且只能承受较低强度的力。这项研究表明，心肌细胞的收缩行为可以通过循环纳米机械刺激进行机械调节，这种调节的程度和模式取决于细胞连接性和基质机械性能。
The ability to modulate cardiomyocyte contractility is important for bioengineering applications ranging from heart disease treatments to biorobotics. In this study, we examined the changes in contraction frequency of neonatal rat cardiomyocytes upon single-cell-level nanoscale mechanical stimulation using atomic force microscopy. To measure the response of same density of cells, they were micropatterned into micropatches of fixed geometry. To examine the effect of the substrate stiffness on the behavior of cells, they were cultured on a stiffer and a softer surface, glass and poly (dimethylsiloxane), respectively. Upon periodic cyclic stimulation of 300 nN at 5 Hz, a significant reduction in the rate of synchronous contraction of the cell patches on poly(dimethylsiloxane) substrates was observed with respect to their spontaneous beat rate, while the cell patches on glass substrates maintained or increased their contraction rate after the stimulation. On the other hand, single cells mostly maintained their contraction rate and could only withstand a lower magnitude of forces compared to micropatterned cell patches. This study reveals that the contraction behavior of cardiomyocytes can be modulated mechanically through cyclic nanomechanical stimulation, and the degree and mode of this modulation depend on the cell connectivity and substrate mechanical properties.
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DOI：
10.1152/ajpheart.00564.2003
发表时间：
2004-08-01
影响因子：
4.8
作者：
Lieber, SC;Aubry, N;Vatner, SF
通讯作者：
Vatner, SF
DOI：
10.1063/1.4915500
发表时间：
2015-03
期刊：
The Review of scientific instruments
影响因子：
--
作者：
A. Gaitas;R. Malhotra;Tao Li;T. Herron;J. Jalife
通讯作者：
A. Gaitas;R. Malhotra;Tao Li;T. Herron;J. Jalife
影响因子：
17.1
作者：
Shin, Su Ryon;Jung, Sung Mi;Zalabany, Momen;Kim, Keekyoung;Zorlutuna, Pinar;Kim, Sang Bok;Nikkhah, Mehdi;Khabiry, Masoud;Azize, Mohamed;Kong, Jing;Wan, Kai-tak;Palacios, Tomas;Dokmeci, Mehmet R.;Bae, Hojae;Tang, Xiaowu (Shirley);Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
3.4
作者：
Rodriguez, Anthony G.;Han, Sangyoon J.;Sniadecki, Nathan J.
通讯作者：
Sniadecki, Nathan J.
影响因子：
5
作者：
Thompson, Susan A.;Blazeski, Adriana;Copeland, Craig R.;Cohen, Daniel M.;Chen, Christopher S.;Reich, Daniel M.;Tung, Leslie
通讯作者：
Tung, Leslie