Carbon-nanotube-embedded hydrogel sheets for engineering cardiac constructs and bioactuators.
Carbon-nanotube-embedded hydrogel sheets for engineering cardiac constructs and bioactuators.
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DOI：
10.1021/nn305559j
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发表时间：
2013-03-26
期刊：
影响因子：
17.1
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Shin, Su Ryon;Jung, Sung Mi;Zalabany, Momen;Kim, Keekyoung;Zorlutuna, Pinar;Kim, Sang Bok;Nikkhah, Mehdi;Khabiry, Masoud;Azize, Mohamed;Kong, Jing;Wan, Kai-tak;Palacios, Tomas;Dokmeci, Mehmet R.;Bae, Hojae;Tang, Xiaowu (Shirley);Khademhosseini, Ali
我们通过将新生大鼠心肌细胞接种到碳纳米管（CNT）结合光交联明胶甲基丙烯酸酯（GelMA）水凝胶上来设计功能性心脏贴片。由此产生的心脏结构表现出良好的机械完整性和先进的电生理功能。具体而言，与在原始GelMA水凝胶上培养的心肌组织相比，在50 μm厚的CNT-GelMA上培养的心肌组织显示出3倍高的自发同步搏动率和85%低的兴奋阈值。我们的研究结果表明，在多孔明胶框架内由CNT形成的导电和纳米纤维网络是CNT-GelMA的关键特征，从而改善心脏细胞粘附、组织和细胞-细胞耦合。厘米级的补丁从玻璃基板上释放，形成3D生物混合致动器，它显示出可控的线性循环收缩/伸展，泵送，和游泳致动。此外，我们首次证明了在CNT-GelMA上培养的心脏组织抵抗模型心脏抑制剂以及细胞毒性化合物的损伤。因此，将碳纳米管掺入明胶和潜在的其他生物材料中，可以用于创建用于治疗目的和体外研究的多功能心脏支架。这些混合材料也可以用于神经元和其他肌肉细胞，以创建具有改善的组织、电活性和机械完整性的组织结构。
We engineered functional cardiac patches by seeding neonatal rat cardiomyocytes onto carbon nanotube (CNT) incorporated photocrosslinkable gelatin methacrylate (GelMA) hydrogel. The resulting cardiac constructs showed excellent mechanical integrity and advanced electrophysiological functions. Specifically, myocardial tissues cultured on 50 μm thick CNT-GelMA showed 3 times higher spontaneous synchronous beating rates and 85% lower excitation threshold, compared to those cultured on pristine GelMA hydrogels. Our results indicate that the electrically conductive and nanofibrous networks formed by CNTs within a porous gelatin framework is the key characteristics of CNT-GelMA leading to improved cardiac cell adhesion, organization, and cell-cell coupling. Centimeter-scale patches were released from glass substrates to form 3D biohybrid actuators, which showed controllable linear cyclic contraction/extension, pumping, and swimming actuations. In addition, we demonstrate for the first time that cardiac tissues cultured on CNT-GelMA resist damage by a model cardiac inhibitor as well as a cytotoxic compound. Therefore, incorporation of CNTs into gelatin, and potentially other biomaterials, could be useful in creating multifunctional cardiac scaffolds for both therapeutic purposes and in vitro studies. These hybrid materials could also be used for neuron and other muscle cells to create tissue constructs with improved organization, electroactivity, and mechanical integrity.
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影响因子：
14
作者：
Nichol, Jason W.;Koshy, Sandeep T.;Bae, Hojae;Hwang, Chang M.;Yamanlar, Seda;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
14
作者：
Aubin H;Nichol JW;Hutson CB;Bae H;Sieminski AL;Cropek DM;Akhyari P;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
3.8
作者：
Bhana, Bashir;Iyer, Rohin K.;Radisic, Milica
通讯作者：
Radisic, Milica
影响因子：
3.7
作者：
Galano, Annia
通讯作者：
Galano, Annia
影响因子：
2.4
作者：
Garzon, Alejandro;Grigoriev, Roman O.;Fenton, Flavio H.
通讯作者：
Fenton, Flavio H.