Real-Time, In Vivo Molecular Monitoring Using Electrochemical Aptamer Based Sensors: Opportunities and Challenges.
Real-Time, In Vivo Molecular Monitoring Using Electrochemical Aptamer Based Sensors: Opportunities and Challenges.
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DOI：
10.1021/acssensors.2c01428
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发表时间：
2022-10-28
期刊：
影响因子：
8.9
通讯作者：
Plaxco, Kevin W.
中科院分区：
文献类型：
--
作者：
Downs, Alex M.;Plaxco, Kevin W.
关键词：
对体内特定分子的连续、实时测量将极大地提高我们理解、诊断和治疗疾病的能力。然而，绝大多数连续分子传感技术要么（1）依赖于其目标的化学或酶反应性，从而极大地限制了它们的范围，要么（2）从未被证明（并且可能永远不会被证明）可以在体内发现的复杂环境中运行。在此背景下，我们在此回顾基于电化学适配体 (EAB) 的传感器，这是一种用于实时分子监测的电化学方法，现已经历了 15 年的学术发展。 EAB 平台的优势非常显着：迄今为止，它是唯一具有以下特点的分子测量技术：(1) 其功能独立于目标的化学反应性，因此具有通用性；(2) 支持体内测量。具体来说，我们和其他人已经使用 EAB 传感器对活体动物的静脉和实体组织中多种不相关的药物和代谢物进行了实时、秒级分辨的测量。针对这些优势，我们详细介绍了该平台的其余弱点，其中包括仍然有限的测量持续时间（数小时，而不是更理想的天数）以及针对新目标获得足够高性能的适体的困难，然后详细介绍克服这些障碍的有希望的方法。最后，我们最后探讨了我们相信这项潜在革命性技术（以及一些可能具有竞争性的技术）将为研究人员和临床医生创造的机会。
The continuous, real-time measurement of specific molecules in situ in the body would greatly improve our ability to understand, diagnose, and treat disease. The vast majority of continuous molecular sensing technologies, however, either (1) rely on the chemical or enzymatic reactivity of their targets, sharply limiting their scope, or (2) have never been shown (and likely will never be shown) to operate in the complex environments found in vivo. Against this background, here we review electrochemical aptamer-based (EAB) sensors, an electrochemical approach to real-time molecular monitoring that has now seen 15 years of academic development. The strengths of the EAB platform are significant: to date it is the only molecular measurement technology that (1) functions independently of the chemical reactivity of its targets, and is thus general, and (2) supports in vivo measurements. Specifically, using EAB sensors we, and others, have already reported the real-time, seconds-resolved measurements of multiple, unrelated drugs and metabolites in situ in the veins and solid tissues of live animals. Against these strengths, we detail the platform’s remaining weaknesses, which include still limited measurement duration (hours, rather than the more desirable days) and the difficulty in obtaining sufficiently high-performance aptamers against new targets, before then detailing promising approaches overcoming these hurdles. Finally, we close by exploring the opportunities we believe this potentially revolutionary technology (as well as a few, possibly competing technologies) will create for both researchers and clinicians.
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DOI：
10.3390/s120911922
发表时间：
2012
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Cash KJ;Clark HA
通讯作者：
Clark HA
影响因子：
3.9
作者：
Brothers, Michael C.;Moore, David;Kim, Steve S.
通讯作者：
Kim, Steve S.
影响因子：
5
作者：
Ferreira, Nuno R.;Ledo, Ana;Barbosa, Rui M.
通讯作者：
Barbosa, Rui M.
影响因子：
--
作者：
Arroyo-Curras, Netzahualcoyotl;Ortega, Gabriel;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.
影响因子：
48
作者：
Clark, Jeremy J.;Sandberg, Stefan G.;Wanat, Matthew J.;Gan, Jerylin O.;Horne, Eric A.;Hart, Andrew S.;Akers, Christina A.;Parker, Jones G.;Willuhn, Ingo;Martinez, Vicente;Evans, Scott B.;Stella, Nephi;Phillips, Paul E. M.
通讯作者：
Phillips, Paul E. M.