Porphyromonas gingivalis induces CCR5-dependent transfer of infectious HIV-1 from oral keratinocytes to permissive cells.
Porphyromonas gingivalis induces CCR5-dependent transfer of infectious HIV-1 from oral keratinocytes to permissive cells.
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DOI：
10.1186/1742-4690-5-29
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发表时间：
2008-03-27
期刊：
影响因子：
3.3
通讯作者：
Herzberg, Mark C.
中科院分区：
文献类型：
--
作者：
Giacaman, Rodrigo A.;Asrani, Anil C.;Gebhard, Kristin H.;Dietrich, Elizabeth A.;Vacharaksa, Anjalee;Ross, Karen F.;Herzberg, Mark C.
通过口服途径感染HIV的情况很少发生。发生的频率可能会增加伴随细菌感染的口腔组织，因为共同感染和炎症的一些细胞类型增加HIV-1复制。牙龈卟啉单胞菌是一种公认的牙周致病菌，它选择性地上调口腔角质形成细胞上HIV-1辅助受体CCR 5的表达。因此，我们假设牙龈卟啉单胞菌调节口腔上皮细胞中HIV感染的结果。将口腔和扁桃体上皮细胞与牙龈卟啉单胞菌预孵育，并接种X4型或R5型HIV-1。在接种后6至48小时之间，牙龈卟啉单胞菌选择性地增加了来自口腔和扁桃体角质形成细胞的R5嗜性HIV-1的感染性; X4嗜性HIV-1的感染性保持不变。口腔角质形成细胞似乎窝藏传染性HIV-1，没有证据表明生产性感染。HIV-1在HIV暴露后的前6小时内处于最高水平，并在48小时降至几乎检测不到的水平。HIV似乎没有与牙龈卟啉单胞菌共定位，牙龈卟啉单胞菌增加了从角质形成细胞到允许细胞的选择性R5嗜性HIV-1反式感染。当选择性阻断CCR 5时，HIV-1反式感染减少。牙龈卟啉单胞菌上调CCR 5增加了携带R5-嗜性HIV-1从口腔角质形成细胞到允许细胞的反式感染。因此，口腔感染（如牙周炎）可能会增加口腔感染和R5嗜性HIV-1传播的风险。
Systemic infection with HIV occurs infrequently through the oral route. The frequency of occurrence may be increased by concomitant bacterial infection of the oral tissues, since co-infection and inflammation of some cell types increases HIV-1 replication. A putative periodontal pathogen, Porphyromonas gingivalis selectively up-regulates expression of the HIV-1 coreceptor CCR5 on oral keratinocytes. We, therefore, hypothesized that P. gingivalis modulates the outcome of HIV infection in oral epithelial cells. Oral and tonsil epithelial cells were pre-incubated with P. gingivalis, and inoculated with either an X4- or R5-type HIV-1. Between 6 and 48 hours post-inoculation, P. gingivalis selectively increased the infectivity of R5-tropic HIV-1 from oral and tonsil keratinocytes; infectivity of X4-tropic HIV-1 remained unchanged. Oral keratinocytes appeared to harbor infectious HIV-1, with no evidence of productive infection. HIV-1 was harbored at highest levels during the first 6 hours after HIV exposure and decreased to barely detectable levels at 48 hours. HIV did not appear to co-localize with P. gingivalis, which increased selective R5-tropic HIV-1 trans infection from keratinocytes to permissive cells. When CCR5 was selectively blocked, HIV-1 trans infection was reduced. P. gingivalis up-regulation of CCR5 increases trans infection of harbored R5-tropic HIV-1 from oral keratinocytes to permissive cells. Oral infections such as periodontitis may, therefore, increase risk for oral infection and dissemination of R5-tropic HIV-1.
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影响因子：
64.5
作者：
Geijtenbeek, TBH;Torensma, R;Figdor, CG
通讯作者：
Figdor, CG
影响因子：
3.8
作者：
Delezay, O;Koch, N;Fantini, J
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影响因子：
5.4
作者：
Daecke, J;Fackler, OT;Kräusslich, HG
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Kräusslich, HG
影响因子：
4.4
作者：
Equils, O;Faure, E;Arditi, M
通讯作者：
Arditi, M
影响因子：
4.4
作者：
Giacaman, Rodrigo A.;Nobbs, Angela H.;Herzberg, Mark C.
通讯作者：
Herzberg, Mark C.