Concomitant enhancement of the response to Mls‐la antigens and the induction of post‐thymectomy autoimmune gastritis in BALB/c mice
Concomitant enhancement of the response to Mls‐la antigens and the induction of post‐thymectomy autoimmune gastritis in BALB/c mice
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BALB/c 小鼠对 Mls-1a 抗原的反应同时增强并诱导胸腺切除后自身免疫性胃炎
DOI：
10.1111/j.1365-2249.1993.tb03428.x
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发表时间：
1993
影响因子：
4.6
通讯作者：
T. Masuda
中科院分区：
文献类型：
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作者：
K. Murakami;M. Hosono;H. Maruyama;Y. Mori;A. Nishio;M. Fukumoto;Y. Watanabe;M. Inaba;K. Kuribayashi;M. Saka;T. Masuda
我们研究了Mis抗原在胸腺摘除BALB/c和DBA/2小鼠诱发自身免疫性胃炎(AIG)中的作用。在出生后第3天行胸腺切除(3D-TX)的MLS-1b小鼠中，AIG的患病率为78%，而在Mis-1a DBA/2小鼠中，AIG的患病率为6%。而ALG阴性的3D-TX DBA/2小鼠产生2-巯基乙醇(2-MF)敏感的抗壁细胞自身抗体。AIG阳性的BALB/c小鼠产生2-ME抗性的抗壁细胞自身抗体。此外，在出生后24小时内通过注射(BALB/c±DBA/2)F1小鼠的骨髓细胞而对M IS-1a抗原产生耐受性的3D-Tx BALB/c小鼠的AIG患病率比非耐受对照组小鼠降低：对照组和耐受组小鼠的AIG患病率分别为80%和30%。因此，辅助T细胞的激活，包括对MLS-1a抗原反应的T细胞和包括免疫球蛋白类开关在内的T细胞，似乎与AIG的诱导密切相关。流式细胞仪分析证实，急性免疫球蛋白小鼠C‘D4-Vβ6T细胞在胃局部淋巴结内明显增多。然而，已知在3D-TX小鼠中增加的Vβll T细胞的数量在CD8T细胞群中发生了增加，但在CD4T细胞群中没有出现。将摩押注射到L3T4。但不是Lyt2。V-β6-或V-β8-Tcr导入3D-Tx BALB/c和接受3D-Tx BALB/c荷瘤小鼠脾细胞的同基因裸鼠，完全抑制了AIG的形成。尽管所有小鼠中表达与注射抗体相关的标志的T细胞显着减少。提示AIG小鼠体内VbT6+L3T4+T细胞的增加伴随着A IG诱导的Vβ6-L3T4+T细胞的激活。
We examined the role of Mis antigens in the induction of autoimmune gastritis (AIG) in BALB/c and DBA/2 mice subjected to thymeclomy. The prevalence of AIG in Mls‐lb mice which underwent thymectomy on day 3 after birth (3d‐Tx) was 78% (mean), while in Mis‐1a DBA/2 mice it was < 6%. Whereas AlG‐negative 3d‐Tx DBA/2 mice produced 2‐mercaptoethanol (2‐MF.)–sensitive anti‐parietal cell autoantibody. AIG‐positive BALB/c mice made 2–ME‐resistant anti‐parietal cell autoantibody. In addition, the prevalence of AIG in 3d‐Tx BALB/c mice which were rendered tolerant to M Is‐1a antigens by injection of bone marrow cells from (BALB/c ± DBA/2)F1 mice within 24 h after birth was decreased compared with the non‐tolerant control mice: the prevalence being 80% in the controls and 30% in the tolerant animals. Thus, the activation of helper T cells, including T cells responding to Mls‐1a antigens and including immunoglobulin class switch, appeared to be closely associated with the induction of AIG. Flowcytometric analysis confirmed that C'D4‐ Vβ6 T cells had increased in the regional lymph nodes of the stomach in AIG mice. However, an increase in the number of Vβll T cells, which are known to increase in 3d‐Tx mice, occurred in the CD8, but not in the CD4 T cell population. Injection of MoAb to L3T4. but not Lyt2. Vβ6‐or Vβ8–TCR, into 3d‐Tx BALB/c and syngeneic nude mice which had received spleen cells of 3d‐Tx BALB/c mice bearing AIG completely abrogated the development of AIG. despite there being remarkable decreases in T cells expressing relevant markers to the injected antibodies in all the mice. These findings suggest that the increase of VbT6+L3T4+ T cells in AIG mice was concomitant with the activation of A IG‐inducing Vβ6‐ L3T4+ T cells.
通过甲状腺炎和过敏性脑脊髓炎的实验模型分析自身免疫。
影响因子：
--
作者：
Weigle,WO
通讯作者：
Weigle,WO
影响因子：
56.9
作者：
H. Smith;I. Chen;R. Kubo;KS K Tung
通讯作者：
H. Smith;I. Chen;R. Kubo;KS K Tung