Low-power laser irradiation promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic adenosine monophosphate.
Low-power laser irradiation promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic adenosine monophosphate.
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DOI：
10.1038/ijos.2013.38
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发表时间：
2013-06
影响因子：
14.9
通讯作者：
Wang, Yan-Hsiung
中科院分区：
文献类型：
--
作者：
Wu, Jyun-Yi;Chen, Chia-Hsin;Yeh, Li-Yin;Yeh, Ming-Long;Ting, Chun-Chan;Wang, Yan-Hsiung
关键词：
保持或改善牙周膜（PDL）的功能是修复牙周缺损的关键。本研究的目的是评估低功率激光照射（LPLI）对人牙周膜细胞（hPDL）增殖和成骨分化的生理效应。每天以0、1、2或4 J·cm-2的剂量照射（660 nm）培养的hPDL细胞。采用MTT法检测细胞增殖，茜素红S染色和碱性磷酸酶（ALP）活性检测LPLI对成骨分化的影响。此外，成骨标志物基因表达通过实时逆转录-聚合酶链反应（RT-PCR）证实。我们的数据显示，2 J·cm-2剂量的LPLI在第3天和第5天显著促进hPDL细胞增殖。此外，能量剂量为2和4 J·cm-2的LPLI显示出潜在的成骨能力，因为它刺激ALP活性、钙沉积和成骨基因表达。我们还表明，环磷酸腺苷（cAMP）是一个关键的调节剂的LIFE介导的作用对hPDL细胞。本研究表明LPLI能促进hPDL细胞的增殖和成骨分化。这些结果表明LPLI在牙周组织再生的临床应用中具有潜在的用途。
Retaining or improving periodontal ligament (PDL) function is crucial for restoring periodontal defects. The aim of this study was to evaluate the physiological effects of low-power laser irradiation (LPLI) on the proliferation and osteogenic differentiation of human PDL (hPDL) cells. Cultured hPDL cells were irradiated (660 nm) daily with doses of 0, 1, 2 or 4 J⋅cm−2. Cell proliferation was evaluated by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, and the effect of LPLI on osteogenic differentiation was assessed by Alizarin Red S staining and alkaline phosphatase (ALP) activity. Additionally, osteogenic marker gene expression was confirmed by real-time reverse transcription-polymerase chain reaction (RT-PCR). Our data showed that LPLI at a dose of 2 J⋅cm−2 significantly promoted hPDL cell proliferation at days 3 and 5. In addition, LPLI at energy doses of 2 and 4 J⋅cm−2 showed potential osteogenic capacity, as it stimulated ALP activity, calcium deposition, and osteogenic gene expression. We also showed that cyclic adenosine monophosphate (cAMP) is a critical regulator of the LPLI-mediated effects on hPDL cells. This study shows that LPLI can promote the proliferation and osteogenic differentiation of hPDL cells. These results suggest the potential use of LPLI in clinical applications for periodontal tissue regeneration.
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影响因子：
2.1
作者：
Bernardes da Silva, Ana Paula R.;Petri, Alice D.;Sasso Stuani, Maria Bernadete
通讯作者：
Sasso Stuani, Maria Bernadete
影响因子：
4.1
作者：
Doorn, Joyce;Leusink, Maarten;de Boer, Jan
通讯作者：
de Boer, Jan
影响因子：
4.3
作者：
BOWERS, GM;CHADROFF, B;ROMBERG, E
通讯作者：
ROMBERG, E
影响因子：
3.6
作者：
Lee, Joo-Hee;Um, Soyoun;Seo, Byoung Moo
通讯作者：
Seo, Byoung Moo
影响因子：
5.3
作者：
Inada, A;Hamamoto, Y;Seino, Y
通讯作者：
Seino, Y