Small intestinal submucosa seeded with intestinal smooth muscle cells in a rodent jejunal interposition model.
Small intestinal submucosa seeded with intestinal smooth muscle cells in a rodent jejunal interposition model.
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在啮齿动物空肠插入模型中接种肠平滑肌细胞的小肠粘膜下层。
DOI：
10.1016/j.jss.2011.08.001
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Dunn,JamesCY
中科院分区：
文献类型：
--
作者：
Qin,HarryH;Dunn,JamesCY
小肠粘膜下层（SIS）是一种猪源性的、无细胞的、以胶原为基础的基质，已经在没有种子平滑肌细胞（SMCs）的情况下进行了肠组织工程试验。我们检测了SMCs植入体内啮齿类动物模型后SIS收缩蛋白的表达模式。材料与方法从Lewis大鼠幼崽中分离肠道SMCs。将四层管状smcs种子SIS或空白SIS支架植入成年大鼠空肠介入模型。受者于植入后2、4和8周处死。使用抗SMCs收缩蛋白的抗体检测回收的标本。结果体外培养的小肠SMCs表达α-平滑肌肌动蛋白（α-SMA）、钙钙蛋白和较少的平滑肌肌球蛋白重链（SM-MHC）。植入细胞种子的SIS支架在8周内明显收缩，但与未植入细胞种子的SIS支架相当。植入细胞种子的SIS支架在2周时表达广泛的α-SMA、部分钙钙蛋白和少量的SM-MHC。4周时，表达α- sma的细胞数量明显减少，而表达calponin或SM-MHC的细胞数量很少。8周时出现少量表达α- sma的细胞，而在吻合界面附近出现更多表达钙钙蛋白或SM-MHC的细胞。结论细胞植入后，SIS明显收缩，但收缩蛋白的表达存在于SIS植入部位。植入部位未形成有组织的平滑肌。需要一种更好的支架设计来产生有结构的平滑肌。
BACKGROUNDSmall intestinal submucosa (SIS) is a porcine-derived, acellular, collagen-based matrix that has been tested without seeded smooth muscle cells (SMCs) for intestinal tissue engineering. We examined the expression patterns of contractile proteins of SIS with SMCs implanted in an in vivo rodent model.MATERIALS AND METHODSIntestinal SMCs were isolated from Lewis rat pups. Four-ply tubular SMCs-seeded SIS or blank SIS scaffolds were implanted in an adult rat jejunal interposition model. Recipients were sacrificed at 2, 4, and 8 wk following the implantation. The retrieved specimens were examined using antibodies against contractile proteins of SMCs.RESULTSCultured intestinal SMCs expressed α-smooth muscle actin (α-SMA), calponin, and less smooth muscle myosin heavy chain (SM-MHC) in vitro. Cell-seeded SIS scaffolds contracted significantly over 8 wk of implantation but were comparable to SIS scaffolds without cell seeding. Implanted cell-seeded SIS scaffolds at 2 wk expressed extensive α-SMA, some calponin, and minimal SM-MHC. At 4 wk, α-SMA-expressing cells decreased significantly, whereas calponin or SM-MHC expressing cells were rarely detected. A small number of α-SMA-expressing cells were present at 8 wk, whereas more calponin or SM-MHC expressing cells emerged in proximity with the anastomotic interface.CONCLUSIONSCell-seeded SIS contracted significantly after implantation, but the expressions of contractile proteins were present at the site of SIS interposition. No organized smooth muscle was formed at the site of implantation. A better scaffold design is needed to produce structured smooth muscle.
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DOI：
10.1172/jci109865
发表时间：
1980
期刊：
The Journal of clinical investigation
影响因子：
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影响因子：
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DOI：
10.1172/jci105436
发表时间：
1966
期刊：
The Journal of clinical investigation
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