Two-photon intravital multicolour imaging to study metastatic behaviour of cancer cells in vivo.
Two-photon intravital multicolour imaging to study metastatic behaviour of cancer cells in vivo.
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双光子活体多色成像研究癌细胞体内转移行为。
DOI：
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发表时间：
2011
影响因子：
--
通讯作者：
B. van de Water
中科院分区：
文献类型：
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作者：
Sylvia E. Le Dévédec;Wies van Roosmalen;C. Pont;R. Lalai;H. D. de Bont;B. van de Water
在过去的十年中，在单细胞分辨率下对小鼠乳腺肿瘤的活体显微镜研究已经导致了对转移行为机制的重要新见解，如肿瘤细胞的迁移、侵袭和内浸润；血管生成;免疫细胞的反应。本章描述了可用于分析乳腺癌转移小鼠模型中肿瘤细胞运动的方法。它包括标记原发肿瘤的生成、双光子显微镜成像和延时图像数据处理的协议。此外，我们提出了一种方法，该方法最近在我们的实验室开发，将多色成像与诱导细胞模型相结合，以研究特定基因在体内肿瘤细胞运动中的作用。该方案可用于成像不同个体肿瘤细胞在同一肿瘤微环境中的转移行为，并将其与转移形成联系起来。还包括标记巨噬细胞以显示血流和图像分析的附加方案。
In the last decade, intravital microscopy on breast tumours in mice at single-cell resolution has resulted in important new insight into mechanisms of metastatic behaviour such as migration, invasion, and intravasation of tumour cells; angiogenesis; and the response of immune cells. This chapter describes the methods that can be used for analysing tumour cell motility in a mouse model of breast cancer metastasis. It includes protocols for generation of a labelled primary tumour, its imaging with two-photon microscopy, and the processing of time-lapse image data. Furthermore, we present a methodology, recently developed in our laboratory that combines multicolour imaging with an inducible cell model to study the role of a specific gene of interest in tumour cell motility in vivo. This protocol can be used to image the metastatic behaviour of different individual tumour cells within the same tumour microenvironment and correlate it with metastasis formation. Additional protocols for labelling macrophages to visualise blood flow and image analysis are also included.
影响因子：
8.4
作者：
Condeelis, JS;Wyckoff, J;Segall, JE
通讯作者：
Segall, JE