Fine Mapping of qd1, a Dominant Gene that Regulates Stem Elongation in Bread Wheat.
Fine Mapping of qd1, a Dominant Gene that Regulates Stem Elongation in Bread Wheat.
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DOI：
10.3389/fgene.2021.793572
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发表时间：
2021
影响因子：
3.7
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Xie Y;Zeng W;Wang C;Xu D;Guo H;Xiong H;Fang H;Zhao L;Gu J;Zhao S;Ding Y;Liu L
茎伸长是决定产量的关键阶段，也是面包小麦（Triticum aestivum L.）改良的主要性状。在之前的研究中，我们描述了一个突变体，它表现出快速的茎伸长，但对成熟时的植株高度没有影响。目前的研究旨在精细地绘制出该突变体中潜在的突变基因qd1。通过对一个由606个个体组成的F2分离群体的分析，我们发现qd1基因表现为显性。此外，通过基于BSR-seq的连锁分析方法，我们初步将qd1基因定位在4B染色体上13.55 Mb的区域（从15.41到28.96 Mb）。这一结果在F2和BC3F2分离群体中得到进一步证实。此外,利用转录组测序数据,我们开发了14 Kompetitive Allele-Specific PCR (KASP)标记然后qd1基因映射到一个更小、更精确的5.08 Mb间隔从26.80到31.88 Mb。开发额外的标记精细地图qd1基因,共有4481个单核苷酸多态性(snp)在5.08 Mb区间筛选,和25 KASP标记开发基于10 x-depth基因组重测序数据从野生型(WT)和突变体植物。qd1基因最终定位在4B染色体28.86 ~ 30.19 Mb的1.33 Mb区间。在该区域鉴定出4个候选基因。其中，只有TraesCS4B02G042300基因在茎中的表达模式与突变体和WT的茎发育是同步的，qd1基因可以与分子标记联合使用来操纵茎发育。
Stem elongation is a critical phase for yield determination and, as a major trait, is targeted for manipulation for improvement in bread wheat (Triticum aestivum L.). In a previous study, we characterized a mutant showing rapid stem elongation but with no effect on plant height at maturity. The present study aimed to finely map the underlying mutated gene, qd1, in this mutant. By analyzing an F2 segregating population consisting of 606 individuals, we found that the qd1 gene behaved in a dominant manner. Moreover, by using the bulked segregant RNA sequencing (BSR-seq)-based linkage analysis method, we initially mapped the qd1 gene to a 13.55 Mb region on chromosome 4B (from 15.41 to 28.96 Mb). This result was further confirmed in F2 and BC3F2 segregating populations. Furthermore, by using transcriptome sequencing data, we developed 14 Kompetitive Allele-Specific PCR (KASP) markers and then mapped the qd1 gene to a smaller and more precise 5.08 Mb interval from 26.80 to 31.88 Mb. To develop additional markers to finely map the qd1 gene, a total of 4,481 single-nucleotide polymorphisms (SNPs) within the 5.08 Mb interval were screened, and 25 KASP markers were developed based on 10x-depth genome resequencing data from both wild-type (WT) and mutant plants. The qd1 gene was finally mapped to a 1.33 Mb interval from 28.86 to 30.19 Mb on chromosome 4B. Four candidate genes were identified in this region. Among them, the expression pattern of only TraesCS4B02G042300 in the stems was concurrent with the stem development of the mutant and WT. The qd1 gene could be used in conjunction with molecular markers to manipulate stem development in the future.
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影响因子：
6.9
作者：
Borràs-Gelonch G;Rebetzke GJ;Richards RA;Romagosa I
通讯作者：
Romagosa I
影响因子：
5.6
作者：
Guo Z;Chen D;Schnurbusch T
通讯作者：
Schnurbusch T
DOI：
10.1071/pp00021
发表时间：
2000-01-01
期刊：
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY
影响因子：
--
作者：
Miralles, DJ;Richards, RA;Slafer, GA
通讯作者：
Slafer, GA
影响因子：
6.9
作者：
Kronenberg L;Yates S;Boer MP;Kirchgessner N;Walter A;Hund A
通讯作者：
Hund A
影响因子：
64.8
作者：
Peng, JR;Richards, DE;Harberd, NP
通讯作者：
Harberd, NP