ArsV and ArsW provide synergistic resistance to the antibiotic methylarsenite.
ArsV and ArsW provide synergistic resistance to the antibiotic methylarsenite.
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DOI：
10.1111/1462-2920.15817
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发表时间：
2021-12
影响因子：
5.1
通讯作者：
Rosen BP
中科院分区：
文献类型：
--
作者：
Chen J;Zhang J;Wu YF;Zhao FJ;Rosen BP
有毒有机砷进入环境的生物和人为活动，如微生物甲基化的无机砷和五价除草剂，如二甲胂酸（MSMA或MAs（V））。三价MAs（III）的毒性比亚砷酸盐或砷酸盐大得多。微生物已经进化出有机砷解毒的机制。我们以前确定了ArsV，黄素连接的单加氧酶，并证明它赋予耐甲胂的氧化methylarsenate。arsV基因通常位于由ArsR阻遏物控制的抗砷（ars）操纵子中，并与甲基砷外排基因（arsK或推定转运蛋白基因）相邻。在这里，我们表明，Paracoccus sp. SY氧化甲基砷。它有一个ars操纵子，有三个基因，arsR，arsV和一个称为arsW的转运基因。arsV在大肠杆菌中的异源表达赋予了对MAs（III）的抗性，而arsW没有。arsV和arsW的共表达与单独表达相比增加了抗性。细胞氧化甲基砷，积累较少的甲基砷。E.大肠杆菌细胞表达积累的砷化钨。我们建议，ArsV是一个单加氧酶，氧化甲基砷到methylarsenate，这是挤压的ArsW，只有少数已知的五价有机砷外排渗透酶，一种新的途径的有机砷抗性。
Toxic organoarsenicals enter the environment from biogenic and anthropogenic activities such as microbial methylation of inorganic arsenic and pentavalent herbicides such as monosodium methylarsenate (MSMA or MAs(V)). Trivalent MAs(III) is considerably more toxic than arsenite or arsenate. Microbes have evolved mechanisms to detoxify organoarsenicals. We previously identified ArsV, a flavin-linked monooxygenase and demonstrated that it confers resistance to methylarsenite by oxidation to methylarsenate. The arsV gene is usually in an arsenic resistance (ars) operon controlled by an ArsR repressor and adjacent to a methylarsenite efflux gene, either arsK or a gene for a putative transporter. Here we show that Paracoccus sp. SY oxidizes methylarsenite. It has an ars operon with three genes, arsR, arsV and a transport gene termed arsW. Heterologous expression of arsV in Escherichia coli conferred resistance to MAs(III), while arsW did not. Co-expression of arsV and arsW increased resistance compared with either alone. The cells oxidized methylarsenite and accumulated less methylarsenate. Everted membrane vesicles from E. coli cells expressing arsW-accumulated methylarsenate. We propose that ArsV is a monooxygenase that oxidizes methylarsenite to methylarsenate, which is extruded by ArsW, one of only a few known pentavalent organoarsenical efflux permeases, a novel pathway of organoarsenical resistance.
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影响因子：
3.5
作者：
Paez-Espino, David;Tamames, Javier;Canovas, David
通讯作者：
Canovas, David
影响因子：
4.8
作者：
Ordonez, Efren;Thiyagarajan, Saravanamuthu;Rosen, Barry P.
通讯作者：
Rosen, Barry P.
影响因子：
3.6
作者：
Chen J;Yoshinaga M;Garbinski LD;Rosen BP
通讯作者：
Rosen BP
影响因子：
11.4
作者：
Chen J;Rosen BP
通讯作者：
Rosen BP
DOI：
10.1073/pnas.0506836103
发表时间：
2006-02-14
影响因子：
11.1
作者：
Qin, J;Rosen, BP;Rensing, C
通讯作者：
Rensing, C